OXIDATION OF ASCORBATE BY ELECTRON AFFINIC DRUGS AND CARCINOGENS
OXIDATION OF ASCORBATE BY ELECTRON AFFINIC DRUGS AND CARCINOGENS
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DOI：
10.1111/j.1751-1097.1978.tb07034.x
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发表时间：
1978-01-01
影响因子：
3.3
通讯作者：
KOCH, C
中科院分区：
文献类型：
--
作者：
BIAGLOW, JE;JACOBSON, B;KOCH, C
硝基苯类、致癌物4-硝基吡啶-N-氧化物和4-硝基喹啉-N-氧化物以及硝基呋喃类（也已知是致癌物）已被发现可增强抗坏血酸盐与氧气的反应。该反应导致抗坏血酸的氧化，产生脱氢抗坏血酸、超氧自由基、过氧化物和水。药物在氧化过程中不会被还原成稳定的中间体，而是被回收到其原始状态。氧的消耗被超氧化物歧化酶或过氧化氢酶部分抑制。如果反应混合物中同时包含超氧化物歧化酶和过氧化氢酶，则总氧消耗量等于抗坏血酸氧化为脱氢抗坏血酸和氧还原为水的预期量。铁细胞色素C可抑制氧消耗。抗坏血酸通过电子转移产生的半醌、硝基和羟胺自由基可还原铁细胞色素，这些氧活性自由基负责刺激氧利用和抗坏血酸氧化。此外，我们还发现含有过氧化氢酶和超氧化物歧化酶的埃利希细胞抑制药物催化的抗坏血酸氧化。氰化物的存在，已知抑制过氧化氢酶和超氧化物歧化酶，消除了大多数测试药物的细胞效应。
The nitrobenzenes, the carcinogens 4‐nitropyridine‐N‐oxide and 4‐nitro‐quinoline‐N‐oxide as well as the nitrofurans, also known to be carcinogenic, have been found to enhance the reaction of ascorbate with oxygen. The reaction results in the oxidation of ascorbate, the production of dehydroascorbate, superoxide radical, peroxide and water. The drugs are not reduced to stable intermediates during the oxidation but are recycled to their original state. The oxygen consumption is partially inhibited by either superoxide dismutase or catalase. If both superoxide dismutase and catalase are included in the reaction mixture, total oxygen consumption was equal to the amount expected for oxidation of ascorbate to dehydroascorbate and reduction of oxygen to water. The oxygen consumption was inhibited by ferricytochrome c. Semiquinones, nitro and hydroxylamine radicals, produced by electron transfer from ascorbate, reduce ferricytochromec.These oxygen reactive radicals are responsible for the stimulation of oxygen utilization and ascorbate oxidation. In addition we have found that Ehrlich cells, containing catalase and superoxide dismutase, inhibit the drug catalyzed oxidation of ascorbate. The presence of cyanide, known to inhibit catalase and superoxide dismutase, abolished the cell effect for most of the drugs tested.