Concentration-based self-assembly of phycocyanin
Concentration-based self-assembly of phycocyanin
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DOI：
10.1007/s11120-017-0406-7
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发表时间：
2017-10-01
影响因子：
3.7
通讯作者：
Paltiel, Yossi
中科院分区：
文献类型：
--
作者：
Eisenberg, Ido;Harris, Dvir;Paltiel, Yossi
蓝藻光捕获复合物可以改变其结构以应对波动的环境条件。由于细胞环境的复杂性，研究体内结构变化很困难。在体外模仿这个系统也具有挑战性。体内系统高度浓缩，由于高吸收，光学处理类似的体外浓缩样品很困难。在这项研究中，我们使用模拟体内条件的高浓度藻蓝蛋白 (PC) 溶液绘制了蓝藻天线自组装路径。 PC 是从嗜热蓝藻热聚球藻中分离出来的，并通过多种方法进行测量。 PC具有三种寡聚状态：六聚体、三聚体和单体。我们发现，随着 PC 溶液浓度的增加，寡聚状态发生了变化。这种低聚机制可能使光合生物能够使其光捕获系统适应各种环境条件。
Cyanobacteria light-harvesting complexes can change their structure to cope with fluctuating environmental conditions. Studying in vivo structural changes is difficult owing to complexities imposed by the cellular environment. Mimicking this system in vitro is challenging, as well. The in vivo system is highly concentrated, and handling similar in vitro concentrated samples optically is difficult because of high absorption. In this research, we mapped the cyanobacteria antennas self-assembly pathways using highly concentrated solutions of phycocyanin (PC) that mimic the in vivo condition. PC was isolated from the thermophilic cyanobacterium Thermosynechococcus vulcanus and measured by several methods. PC has three oligomeric states: hexamer, trimer, and monomer. We showed that the oligomeric state was changed upon increase of PC solution concentration. This oligomerization mechanism may enable photosynthetic organisms to adapt their light-harvesting system to a wide range of environmental conditions.