Uptake of Catecholamines by a Particulate Fraction of the Adrenal Medulla
Uptake of Catecholamines by a Particulate Fraction of the Adrenal Medulla
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肾上腺髓质颗粒部分对儿茶酚胺的摄取
作者：
N. Kirshner
从牛肾上腺髓质分离的嗜铬颗粒在37 ℃下从等渗介质中摄取C14标记的儿茶酚胺，其速率约为0 ℃下的20倍。Mg++和三磷酸腺苷刺激了摄取三至五倍。利血平（1 × 10- 5 M）引起的90%的抑制摄取;乙二胺四乙酸完全抑制镁和腺苷三磷酸的刺激，抑制可以通过添加过量的Mg++逆转。
Chromaffin granules isolated from beef adrenal medulla took up C14-labeled catecholamines from an isotonic medium at 37�C at a rate approximately 20 times the rate at 0�C. The uptake was stimulated three- to five-fold by Mg++ and adenosine triphosphate. Reserpine (1 x 10-5M) caused a 90 percent inhibition of uptake; ethylenediaminetetraacetic acid completely inhibited the stimulation by magnesium and adenosine triphosphate, and the inhibition could be reversed by addition of excess Mg++.