Transport of human immunoglobulin G and Fc-fusion proteins to chicken egg yolk
Transport of human immunoglobulin G and Fc-fusion proteins to chicken egg yolk
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DOI：
10.1263/jbb.102.518
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发表时间：
2006-12-01
影响因子：
2.8
通讯作者：
Iijima, Shinji
中科院分区：
文献类型：
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作者：
Kawabe, Yoshinori;Naka, Tsutomu;Iijima, Shinji
我们研究了人免疫球蛋白G（IgG）亚类和融合蛋白与Fc区的人IgG的蛋黄，蛋白质注射到母鸡的静脉后的运输。人IgG被有效运输并积累到蛋黄中，而蛋白质在白色中未检测到。在人IgG亚类中，IgG2的转运效率最高。注射的Fc融合蛋白也被转运到蛋黄中。与来自人IgG2的Fc区的融合蛋白相比，与来自人IgG1的Fc区的对应融合蛋白更有效地转运到蛋黄中。本研究表明，在转基因鸡生物反应器中，从卵黄中回收重组抗体和Fc融合蛋白是一种有效的方法。
We examined the transport of human immunoglobulin G (IgG) subclasses and fusion proteins with the Fc region of human IgG to the egg yolk, after the proteins were injected into a vein of hens. Human IgGs were efficiently transported and accumulated into the yolk, whereas the proteins were not detected in the egg white. Among human IgG subclasses, IgG2 was transported most efficiently. Fc-fusion proteins injected were also transported into the yolk. A fusion protein with the Fc region derived from human IgG2 was more efficiently transported into the yolk than the counterpart fusion with the Fc region from human IgG1. This study shows that the recovery of recombinant antibodies and Fc-fusion proteins from the yolk is an effective method in transgenic chicken bioreactors.