Gene expression shifts during perithecium development in Gibberella zeae (anamorph Fusarium graminearum), with particular emphasis on ion transport proteins
Gene expression shifts during perithecium development in Gibberella zeae (anamorph Fusarium graminearum), with particular emphasis on ion transport proteins
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DOI：
10.1016/j.fgb.2007.04.007
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发表时间：
2007-11-01
影响因子：
3
通讯作者：
Trail, Frances
中科院分区：
文献类型：
--
作者：
Hallen, Heather E.;Huebner, Marianne;Trail, Frances
玉米赤霉菌(Gibberella Zeae)是引起小麦赤霉病的主要病原，在世界范围内对小粒小麦造成严重危害。性周期是头枯病流行病学的重要组成部分，因为强制释放的子囊孢子是主要的接种物。最近开发的一种Affymetrix基因芯片，包含了代表所有预测的玉米螟基因的探测集，为研究有性发育过程中的差异基因表达打开了大门。利用GeneChips，在培养中进行了从营养菌丝到具有多隔子囊孢子的成熟果皮的发育时间进程。时间点代表组成成熟的子囊膜的主要细胞类型的发育。在17,830个玉米象中，大多数(78%)在至少一个发育阶段表达；其中12%似乎是性发育所特有的。对162个预测的离子转运蛋白基因进行了详细的分析，因为它们与子囊功能有关。MIRA类铁载体、氯离子通道、P型ATPase和钾转运蛋白的表达模式在发育阶段表现出一定的特殊性。这是第一次对丝状真菌有性发育过程中差异转录积累的全基因组分析。(C)2007 Elsevier Inc.保留所有权利。
Gibberella zeae, the causal agent of Fusarium head blight, is a devastating pathogen of small grains worldwide. The sexual cycle is a crucial component of head blight epidemiology, as forcibly discharged ascospores serve as the primary inoculum. The recent development of an Affymetrix GeneChip containing probesets representative of all predicted genes of G. zeae has opened the door to studies of differential gene expression during sexual development. Using GeneChips, a developmental time course was performed in culture, from vegetative hyphae to mature perithecia with multiseptate ascospores. Time-points represent the development of the major cell types comprising the mature perithecium. The majority of the 17,830 G. zeae probesets, 78%, were expressed during at least one of the developmental stages; 12% of these appear to be specific to sexual development. Analysis of the 162 predicted ion transporter genes is reported in detail, due to their association with perithecium function. Expression patterns of the MirA-type siderophores, chloride channels, P-type ATPases and potassium transporters show some specialization in regard to developmental stage. This is the first whole-genome analysis of differential transcript accumulation during sexual development in a filamentous fungus. (C) 2007 Elsevier Inc. All rights reserved.