A polydopamine-modified optical fiber SPR biosensor using electroless-plated gold films for immunoassays
A polydopamine-modified optical fiber SPR biosensor using electroless-plated gold films for immunoassays
复制标题
聚多巴胺修饰光纤 SPR 生物传感器，使用化学镀金膜进行免疫分析
DOI：
10.1016/j.bios.2015.06.080
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发表时间：
2015-12-15
影响因子：
12.6
通讯作者：
He, Zhimin
中科院分区：
文献类型：
--
作者：
Shi, Se;Wang, Libing;He, Zhimin
本文提出了一种用于光纤表面等离子体共振（SPR）传感器的灵敏、稳定的化学镀金膜，以及一种简便的抗体固定方法。金纳米颗粒均匀地吸附到光纤的表面上，形成厚度约为563 nm的膜。该传感器在折射率为1.333-1.359和1.359-1386的范围内分别具有2054 nm/RIU和3980 nm/RIU的高灵敏度。利用多巴胺（DA）在金膜上的自发聚合反应，制备了聚多巴胺修饰金膜（PDA-Au）表面等离子体共振（SPR）生物传感器。当固定山羊抗人IgG抗体时，FDA-Au表面的共振波长比传统的11-巯基十一烷酸修饰的金膜（MUA-Au）表面的共振波长移动了66.21 nm。此外，PDA-Au表面能够灵敏和选择性地测定低至2 μ g mL（-1）浓度的人IgG，具有0.41 nm/μ g mL（-1）的高灵敏度。与MUA-Au表面相比，FDA-Au表面对人IgG的灵敏度高约4倍，LOD低约7倍。(C)2015 Elsevier B. V.版权所有。
A sensitive and stable electroless-plated gold film for the preparation of an optical fiber surface plasmon resonance (SPR) sensor is presented in this work, together with a facile antibody immobilization method. Gold nanoparticles were uniformly adsorbed onto the surface of an optical fiber forming a film with a thickness of approximately 563 nm. The sensor had a high sensitivity with 2054 nm/RIU and 3980 nm/RIU in the refractive index ranges of 1.333-1.359 and 1.359-1386, respectively. An SPR biosensor was developed based on polydopamine-modified gold film (PDA-Au), which was fabricated by a simple and quick spontaneous polymerization of dopamine (DA) on the gold film. When goat anti-human IgG antibodies were immobilized, the FDA-Au surface had a larger resonant wavelength shift of 66.21 nm compared with the traditional 11-mercaptoundecanoic acid-modified gold film (MUA-Au) surface. In addition, the PDA-Au surface enabled the sensitive and selective determination of human IgG down to a concentration of 2 mu g mL(-1) with a high sensitivity of 0.41 nm per mu g mL(-1). The FDA-Au surface exhibited an approximately four fold higher sensitivity and an about seven fold lower LOD than the MUA-Au surface to human IgG. (C) 2015 Elsevier B.V. All rights reserved.