Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense.
Genomic characterization of sporadic isolates of the dominant clone of Mycobacterium abscessus subspecies massiliense.
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DOI：
10.1038/s41598-021-94789-y
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发表时间：
2021-07-28
影响因子：
4.6
通讯作者：
Wallace RJ Jr
中科院分区：
文献类型：
--
作者：
Davidson RM;Benoit JB;Kammlade SM;Hasan NA;Epperson LE;Smith T;Vasireddy S;Brown-Elliott BA;Nick JA;Olivier KN;Zelazny AM;Daley CL;Strong M;Wallace RJ Jr
最近的研究已经确定了一个优势克隆（克隆1）的分枝杆菌亚种卡氏亚种（M.马赛里ense）与囊性纤维化（CF）患者的高患病率、美国（美国）和英国（英国）的肺部疫情以及巴西的皮肤感染流行病有关。克隆1在美国非CF患者中的患病率以及散发的美国分离株与爆发克隆的关系尚不清楚。我们调查了参考美国分枝杆菌实验室和美国CF相关分枝杆菌分离株的克隆1生物储存库。然后，我们比较了散发性非CF，CF和爆发克隆1分离株之间的基因组变异和抗菌素耐药性（AMR）突变。在参考实验室样本中，57/147（39%）的M.与CF分离株生物储存库中的11/64（17%）相比，Alzliense具有克隆1，包括肺部和肺外感染。核心和泛基因组分析显示，爆发分离株具有类似数量的单核苷酸多态性（SNP）和辅助基因作为散发的美国克隆1分离株。然而，与散发性分离株相比，肺部爆发分离株更可能具有AMR突变。克隆1分离株存在于美国的非CF和CF患者中，但需要进行额外的研究来解决潜在的传播和传播途径。
Recent studies have characterized a dominant clone (Clone 1) of Mycobacterium abscessus subspecies massiliense (M. massiliense) associated with high prevalence in cystic fibrosis (CF) patients, pulmonary outbreaks in the United States (US) and United Kingdom (UK), and a Brazilian epidemic of skin infections. The prevalence of Clone 1 in non-CF patients in the US and the relationship of sporadic US isolates to outbreak clones are not known. We surveyed a reference US Mycobacteria Laboratory and a US biorepository of CF-associated Mycobacteria isolates for Clone 1. We then compared genomic variation and antimicrobial resistance (AMR) mutations between sporadic non-CF, CF, and outbreak Clone 1 isolates. Among reference lab samples, 57/147 (39%) of patients with M. massiliense had Clone 1, including pulmonary and extrapulmonary infections, compared to 11/64 (17%) in the CF isolate biorepository. Core and pan genome analyses revealed that outbreak isolates had similar numbers of single nucleotide polymorphisms (SNPs) and accessory genes as sporadic US Clone 1 isolates. However, pulmonary outbreak isolates were more likely to have AMR mutations compared to sporadic isolates. Clone 1 isolates are present among non-CF and CF patients across the US, but additional studies will be needed to resolve potential routes of transmission and spread.
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影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
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作者：
Feliziani S;Marvig RL;Luján AM;Moyano AJ;Di Rienzo JA;Krogh Johansen H;Molin S;Smania AM
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Smania AM
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9.4
作者：
Brown-Elliott, Barbara A.;Vasireddy, Sruthi;Wallace, Richard J., Jr.
通讯作者：
Wallace, Richard J., Jr.