THE CALCITONIN RECEPTOR ON T-47D BREAST-CANCER CELLS - EVIDENCE FOR GLYCOSYLATION
THE CALCITONIN RECEPTOR ON T-47D BREAST-CANCER CELLS - EVIDENCE FOR GLYCOSYLATION
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DOI：
10.1042/bj2120609
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发表时间：
1983-01-01
影响因子：
4.1
通讯作者：
MARTIN, TJ
中科院分区：
文献类型：
--
作者：
MOSELEY, JM;FINDLAY, DM;MARTIN, TJ
在人乳腺癌细胞系T 47 D中研究了肽激素降钙素受体的糖基性质。进行研究以评估各种凝集素和抗生素衣霉素抑制降钙素与细胞的特异性结合、减少光敏降钙素与大分子受体组分的交联以及影响降钙素对cAMP的刺激的能力。将细胞与低浓度衣霉素预孵育72小时导致总特异性结合减少。80%和降钙素刺激的腺苷酸环化酶减少40%;交联受体组分的形成也受到抑制。麦胚凝集素表现出最显着的抑制总特异性结合和cAMP的生产。然而，光敏降钙素与受体成分的交联完全被这种凝集素抑制。大豆凝集素带来了非常小的减少总的特异性结合，但有更深刻的影响降钙素刺激cAMP的生产和交联的光敏降钙素。伴刀豆球蛋白A和小扁豆凝集素对所有参数都有一定的抑制作用。T 47 D细胞中的降钙素受体与糖基部分相关，其中主要贡献者是N-乙酰基-D-葡萄糖胺残基，但N-乙酰基-D-半乳糖胺和甘露糖残基也相关。
The glycosyl nature of the receptor for the peptide hormone calcitonin was investigated in a human breast cancer cell line, T 47D. Studies were carried out to assess the ability of various lectins and of the antibiotic tunicamycin to inhibit specific binding of calcitonin to the cells, to reduce cross-linking of photoactive calcitonin to a macromolecular receptor component and to influence calcitonin stimulation of cAMP. Pre-incubation of cells with low concentration of tunicamycin for 72 h resulted in a reduction of total specific binding by .apprx. 80% and a 40% reduction in calcitonin-stimulated adenylate cyclase; formation of the cross-linked receptor component was also inhibited. Wheat-germ lectin showed the most marked inhibition of total specific binding and cAMP production. However, cross-linking of photoactive calcitonin to receptor component was totally inhibited by this lectin. Soya-bean lectin brought about very little reduction in total specific binding but had more profound effects on calcitonin-stimulated cAMP production and cross-linking of photoactive calcitonin. Concanavalin A and lentil lectin showed some inhibition of all parameters. The calcitonin receptor in T 47D cells is associated with glycosyl moieties, the major contributors of which are N-acetyl-D-glucosamine residues, but N-acetyl-D-galactosamine and mannose residues are also associated.