Microvascular smooth muscle cell quantitation from scanning electron microscopic preparations.
Microvascular smooth muscle cell quantitation from scanning electron microscopic preparations.
复制标题
通过扫描电子显微镜制剂对微血管平滑肌细胞进行定量。
DOI：
10.1002/ar.1092160315
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Evan,AP
中科院分区：
文献类型：
--
作者：
Gattone2nd,VH;Miller,BG;Evan,AP
A morphometric method to analyze scanning electron micrographs (SEM) of microvascular smooth muscle cells (SMC) is validated using intestinal arterioles. Features easily obtained from these microvasculature preparations are counted (number of tapers and number of complete wraps in the central 87% of the vessel, which is one-third of the vessel's circumference) or measured (vessel diameter and vessel segment length). These data allow the determination of wraps around the vessel per SMC, cell length, and cell width, which are not different from either the values as determined by circumferential examination and quantitation of the vascular segment or an alternative SEM quantitation method (Miller et al., 1986). The method described herein provides a relatively easy way to determine microvascular SMC parameters.
