Identification of a stem cell niche in the zone of Ranvier within the knee joint
Identification of a stem cell niche in the zone of Ranvier within the knee joint
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DOI：
10.1111/j.1469-7580.2009.01115.x
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发表时间：
2009-09-01
影响因子：
2.4
通讯作者：
Lindahl, Anders
中科院分区：
文献类型：
--
作者：
Karlsson, Camilla;Thornemo, Maria;Lindahl, Anders
关节软骨的浅表损伤不能自发地自我修复，并且已经被认为部分是由于关节内缺乏可以到达损伤部位的祖细胞。为了研究关节内是否存在祖细胞，将3个月大的新西兰白色兔连续12天暴露于溴脱氧尿苷（BrdU），然后在第一次BrdU给药后4、6、10、14、28和56天处死。用免疫组化法检测BrdU的存在和祖细胞标志物的定位。BrdU暴露10天后，在骨骺板、朗维尔软骨膜沟和关节软骨的所有区域中大量检测到BrdU阳性细胞，即增殖细胞。洗脱期后，除骨骺板的增殖区外，在膝关节的这些区域中仍存在BrdU阳性细胞，即被认为是祖细胞的细胞。郎维耳软骨膜沟中的细胞进一步对与祖细胞和干细胞龛相关的几种标志物呈阳性，包括Stro-1、Jagged 1和BMPr 1a。我们的结果表明，Ranvier软骨膜沟以及膝关节的关节软骨中存在少量祖细胞。郎维耳软骨膜沟也证明了干细胞龛的特性。
A superficial lesion of the articular cartilage does not spontaneously self-repair and has been suggested to be partly due to lack of progenitor cells within the joint that can reach the site of injury. To study whether progenitor cells are present within the joint, 3-month-old New Zealand white rabbits were exposed to bromodeoxyuridine (BrdU) for 12 consecutive days and were then sacrificed 4, 6, 10, 14, 28 and 56 days after the first BrdU administration. Presence of BrdU and localization of progenitor markers were detected using immunohistochemistry. After 10 days of BrdU exposure, BrdU-positive cells, i.e. proliferating cells, were abundantly detected in the epiphyseal plate, the perichondrial groove of Ranvier, and in all zones of the articular cartilage. After a wash-out period, BrdU-positive cells were still present, i.e. those considered to be progenitor cells, in these regions of the knee except for the proliferative zone of the epiphyseal plate. Cells in the perichondrial groove of Ranvier were further positive for several markers associated with progenitor cells and stem cell niches, including Stro-1, Jagged1, and BMPr1a. Our results demonstrate that a small population of progenitor cells is present in the perichondrial groove of Ranvier as well as within the articular cartilage in the knee. The perichondrial groove of Ranvier also demonstrates the properties of a stem cell niche.