Soft cluster-induced desorption/ionization mass spectrometry: How soft is soft?
Soft cluster-induced desorption/ionization mass spectrometry: How soft is soft?
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DOI：
10.1116/6.0000046
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发表时间：
2020-03-01
期刊：
影响因子：
2.1
通讯作者：
Duerr, Michael
中科院分区：
文献类型：
--
作者：
Portz, Andre;Bomhardt, Karolin;Duerr, Michael
中性团簇诱导的解吸/电离（DINeC）被用作通过质谱（MS）分析易碎生物分子的超软解吸/电离方法。作为测试分子，用DINeC-MS测量糖肽万古霉素，并将所得质谱与电喷雾电离（ESI）、基质辅助激光解吸电离和飞行时间二次离子MS获得的结果进行比较。在基于解吸的技术中，DINeC光谱显示与ESI光谱相当的片段丰度最低。当应用于替考拉宁的MS分析时，进一步证明了DINeC的软解吸性质。
Desorption/ionization induced by neutral clusters (DINeC) is used as an ultrasoft desorption/ionization method for the analysis of fragile biomolecules by means of mass spectrometry (MS). As a test molecule, the glycopeptide vancomycin was measured with DINeC-MS, and resulting mass spectra were compared to the results obtained with electrospray ionization (ESI), matrix assisted laser desorption ionization, and time-of-flight secondary ion MS. Of the desorption-based techniques, DINeC spectra show the lowest abundance of fragments comparable to ESI spectra. The soft desorption nature of DINeC was further demonstrated when applied to MS analysis of teicoplanin.