Heme oxygenase expression in human placenta and placental bed: reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction
Heme oxygenase expression in human placenta and placental bed: reduced expression of placenta endothelial HO-2 in preeclampsia and fetal growth restriction
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DOI：
10.1096/fj.00-0376com
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发表时间：
2001-05-01
期刊：
影响因子：
4.8
通讯作者：
Lyall, F
中科院分区：
文献类型：
--
作者：
Barber, A;Robson, SC;Lyall, F
在这项研究中，我们检验了血红素加氧酶HO-1和HO-2的表达，它们负责一氧化碳的产生，在妊娠合并先兆子痫（PE）和胎儿生长受限（FGR）的胎盘和胎盘床中与对照组妊娠晚期相比减少。采用Western blot（各组各10例）和免疫组化（对照组18例，PE n=19例，FGR n=10例）检测胎盘蛋白表达，采用胎盘床活检标本免疫组化检测绒毛外滋养细胞表达（对照组17例，PE n=19例，FGR n=10例），胎盘匀浆Western blot分析各组间HO-2无总体差异。然而，免疫组织化学分析显示，在两个异常组内皮细胞中HO-2表达减少(PE P
In this study we tested the hypothesis that expression of heme oxygenases HO-1 and HO-2, which are responsible for the production of carbon monoxide, are reduced in the placenta and placental bed of pregnancies complicated by preeclampsia (PE) and fetal growth restriction (FGR) compared with control third-trimester pregnancies. Placental protein expression was determined by Western blotting (n=10 in each group) and immunohistochemistry (controls n=18, PE n=19, FGR n=10), Extravillous trophoblast expression was determined by immunohistochemistry of placental bed biopsy samples (controls n=17, PE n=19, FGR n=10), Western blot analysis of placental homogenates showed no overall differences in HO-2 among groups. However, immunohistochemical analysis showed a reduction in HO-2 expression in endothelial cells in both abnormal groups (PE P