A metabolomics and proteomics study of the adaptation of Staphylococcus aureus to glucose starvation
A metabolomics and proteomics study of the adaptation of Staphylococcus aureus to glucose starvation
复制标题
DOI：
10.1039/c0mb00315h
复制
发表时间：
2011-01-01
影响因子：
--
通讯作者：
Lalk, Michael
中科院分区：
文献类型：
--
作者：
Liebeke, Manuel;Doerries, Kirsten;Lalk, Michael
作为一种多功能病原体，金黄色葡萄球菌可引起多种疾病模式，这些疾病模式不仅受菌株特异性毒力因子库的影响，还受金黄色葡萄球菌生理适应能力的影响。在这里，我们提出了金黄色葡萄球菌中心代谢途径的代谢组学描述，并展示了基于代谢组学和蛋白质组学的联合方法在这一重要病原体的基础研究中的潜力。这项研究提供了500多种蛋白质和94种代谢物在从指数生长到葡萄糖饥饿过渡期间的时间分辨率图像。在葡萄糖过量的情况下，细胞表现出更高水平的参与糖酵解和蛋白质合成的蛋白质，而进入静止期则引发了TCC酶和糖异生酶的增加。代谢酶水平的这些变化与相关代谢物浓度的更显著变化相一致，特别是糖酵解的中间产物和几种氨基酸。
As a versatile pathogen Staphylococcus aureus can cause various disease patterns, which are influenced by strain specific virulence factor repertoires but also by S. aureus physiological adaptation capacity. Here, we present metabolomic descriptions of S. aureus central metabolic pathways and demonstrate the potential for combined metabolomics- and proteomics-based approaches for the basic research of this important pathogen. This study provides a time-resolved picture of more than 500 proteins and 94 metabolites during the transition from exponential growth to glucose starvation. Under glucose excess, cells exhibited higher levels of proteins involved in glycolysis and protein-synthesis, whereas entry into the stationary phase triggered an increase of enzymes of TCC and gluconeogenesis. These alterations in levels of metabolic enzymes were paralleled by more pronounced changes in the concentrations of associated metabolites, in particular, intermediates of the glycolysis and several amino acids.