Equilibrium Conformation of Catenated DNA Networks in Slitlike Confinement
Equilibrium Conformation of Catenated DNA Networks in Slitlike Confinement
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DOI：
10.1021/acsmacrolett.1c00299
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发表时间：
2021-06-25
影响因子：
7.015
通讯作者：
Doyle, Patrick S.
中科院分区：
文献类型：
--
作者：
Soh, Beatrice W.;Doyle, Patrick S.
动植体是由连结的DNA环组成的平面网络，其拓扑结构类似于锁链盔甲。在这项工作中，我们使用单分子实验来探索限制在狭缝中的动粒的构象。我们发现，动泡体的面内尺寸随着限制程度的增加而增加，类似于线性DNA的狭缝限制。动泡体大小随通道高度的变化与2D聚合物的Flory类型方法的比例预测一致。随着限制程度的增加，动质体似乎展开并呈现出更均匀的圆形，与大量观察到的动质体的广泛构象形成对比。
A kinetoplast is a planar network of catenated DNA rings with topology that resembles that of chain mail armor. In this work, we use single-molecule experiments to probe the conformation of kinetoplasts confined to slits. We find that the in-plane size of kinetoplasts increases with degree of confinement, akin to the slitlike confinement of linear DNA. The change in kinetoplast size with channel height is consistent with the scaling prediction from a Flory-type approach for a 2D polymer. With an increase in extent of confinement, the kinetoplasts appear to unfold and take on more uniform circular shapes, in contrast to the broad range of conformations observed for kinetoplasts in bulk.