Dual effect of sodium nitroprusside on potentials recorded from mouse hippocampal slices.
Dual effect of sodium nitroprusside on potentials recorded from mouse hippocampal slices.
复制标题
硝普钠对小鼠海马切片记录的电位的双重影响。
DOI：
10.1016/0006-8993(94)91710-8
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发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Wieraszko,A
中科院分区：
文献类型：
--
作者：
Li,G;Wieraszko,A
本研究的目的是评估硝普钠（SNP）作为一氧化氮（NO）供体在突触可塑性研究中的作用。观察SNP、K3Fe（CN）6和K4Fe（CN）6对小鼠海马脑片诱发电位的影响。当切片暴露于ImM SNP不超过15分钟时，在灌注后观察到群体尖峰的促进。更长的时间，60分钟的切片暴露于1 mM SNP防止这种灌注诱导的放大的潜力，并阻止诱导LTP随后施加高频刺激（HFS）。较低浓度（0.2mM）的SNP没有影响。SNP（1 mM）抑制NMDA组分的人口穗。光灭活，1.0 mM SNP，当应用15分钟，降低了电位，随后诱导LTP。然而，将切片暴露于光失活的1 mM SNP的时间越长，LTP的诱导就越不稳定并被阻断。K3Fe（CN）6和K4Fe（CN）6是结构上与SNP相关但不能产生NO的分子，它们瞬时扩增了群体锋电位及其NMDA组分。我们认为，SNP，这是经常被用作一氧化氮供体，施加了双重抑制和易化作用对海马诱发电位。这些行动的可能机制进行了讨论。
The purpose of this research was to estimate the usefulness of sodium nitroprusside (SNP) as a donor of nitric oxide (NO) in the study of synaptic plasticity. The influence of SNP, K3Fe(CN)6and K4Fe(CN)6on the evoked potential recorded from mouse hippocampal slices was evaluated. When slices were exposed to 1 mM SNP for no longer than 15 min, a facilitation of the population spike was observed following perfusion. Longer, 60 min exposure of the slices to 1 mM SNP prevented this perfusion-induced amplification of the potential and blocked the induction of LTP by subsequently applied high frequency stimulation (HFS). SNP at lower concentrations (0.2 mM) had no effect. SNP (1 mM) suppressed the NMDA component of the population spike. Light inactivated, 1.0 mM SNP, when applied for 15 min, reduced the potential and subsequently induced LTP. However, longer exposure of the slices to the light inactivated 1 mM SNP made the potential unstable and blocked induction of LTP. K3Fe(CN)6and K4Fe(CN)6, molecules structurally related to SNP but unable to generate NO, transiently amplified the population spike and its NMDA component. We suggest that SNP, which is often used as nitric donor, exerts a dual inhibitory and facilitatory action on hippocampal evoked potentials. The possible mechanisms of these actions are discussed.