Comparative studies on species identification of Noctuoidea moths in two nature reserve conservation zones (Beijing, China) using DNA barcodes and thin‐film biosensor chips
Comparative studies on species identification of Noctuoidea moths in two nature reserve conservation zones (Beijing, China) using DNA barcodes and thin‐film biosensor chips
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DOI：
10.1111/1755-0998.12165
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发表时间：
2014-01
影响因子：
7.7
通讯作者：
F. Yang;Zhi-yong Shi;S. L. Bai;Robert D. Ward;Ai-bing Zhang
中科院分区：
文献类型：
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作者：
F. Yang;Zhi-yong Shi;S. L. Bai;Robert D. Ward;Ai-bing Zhang
害虫夜蛾的生物防治需要快速准确的种类鉴定。DNA条形码和薄膜生物传感器芯片是两种受到广泛关注的分子方法。在这里，我们比较了这两种方法的识别数量有限的夜蛾蛾物种。以常用的线粒体基因细胞色素c氧化酶I（动物标准DNA条形码）为基础，设计合成了14条探针，用于北京和河北两个国家级自然保护区共有的14种动物。探针的长度范围为18至27 bp，并被设计为错配探针，以保证探针和非靶序列之间至少有三个碱基的差异。芯片上的结果，肉眼就能检测出来，不需要特殊设备。尽管我们在14种靶标和24种非靶标夜蛾总科物种上测试了所有探针，但未检测到交叉杂交。基于COI序列的38个物种的邻接树给出了38个高度支持的独立组。基于COI基因的DNA条形码和薄膜生物传感器芯片都能够准确识别和区分本研究中的14种目标蛾类。由于其速度快、精度高、成本低，薄膜生物传感器芯片是一种非常实用的物种识别手段。现在，将开发一种更全面的芯片，用于识别更多的夜蛾科蛾，用于害虫控制和生态保护。
Rapid and accurate identification of species is required for the biological control of pest Noctuoidea moths. DNA barcodes and thin‐film biosensor chips are two molecular approaches that have gained wide attention. Here, we compare these two methods for the identification of a limited number of Noctuoidea moth species. Based on the commonly used mitochondrial gene cytochrome c oxidase I (the standard DNA barcode for animal species), 14 probes were designed and synthesized for 14 species shared by two national nature reserves in Beijing and Hebei, China. Probes ranged in length from 18 to 27 bp and were designed as mismatch probes to guarantee that there were at least three base differences between the probe and nontarget sequences. The results on the chip could be detected by the naked eye without needing special equipment. No cross‐hybridizations were detected although we tested all probes on the 14 target and 24 nontarget Noctuoidea species. The neighbour‐joining tree of the 38 species based on COI sequences gave 38 highly supported independent groups. Both DNA barcoding and thin‐film biosensor chips, based on the COI gene, are able to accurately identify and discriminate the 14 targeted moth species in this study. Because of its speed, high accuracy and low cost, the thin‐film biosensor chip is a very practical means of species identification. Now, a more comprehensive chip will be developed for the identification of additional Noctuoidea moths for pest control and ecological protection.