“Micro”-Insulin Radioimmunoassay: Measurement of the Insulin Response during Glucose Tolerance Tests in Guinea Pigs 1
“Micro”-Insulin Radioimmunoassay: Measurement of the Insulin Response during Glucose Tolerance Tests in Guinea Pigs 1
复制标题
“微”胰岛素放射免疫分析：测量豚鼠葡萄糖耐量试验中的胰岛素反应 1
DOI：
10.3181/00379727-163-40783
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发表时间：
1980
影响因子：
--
通讯作者：
W. Fujimoto
中科院分区：
文献类型：
--
作者：
K. Gorray;W. Fujimoto
摘要 提出了一种对豚鼠多次放血收集的小体积血清中胰岛素水平进行放射免疫测定的方法。通过并行性和恢复研究评估了该技术的有效性。该测定用于测量进食、禁食和禁食+再进食动物组中胰岛素对葡萄糖耐量测试的反应。豚鼠在基础条件下的胰岛素水平比其他物种报道的胰岛素水平高 10 倍。 24 小时禁食后，基础血糖和胰岛素水平均显着下降。禁食的豚鼠还表现出葡萄糖耐量受损和胰岛素峰值延迟。这两种异常现象都可以通过重新喂养而逆转。这些结果表明，尽管豚鼠血液中的免疫反应性胰岛素水平异常高，但其对空腹和葡萄糖耐量测试的胰岛素和葡萄糖反应与报道的其他物种相似。
Summary A method is presented for the radioimmunoassay of insulin levels in small volumes of serum collected by multiple bleedings of guinea pigs. The validity of this technique was evaluated by both parallelism and recovery studies. The assay was utilized to measure the insulin response to a glucose tolerance test in fed, fasted, and fasted + refed groups of animals. Insulin levels in guinea pigs under basal conditions were 10-fold higher than those reported for other species. A significant decline in both basal blood glucose and insulin levels occurred following a 24-hr fast. Fasted guinea pigs also demonstrated an impairment of glucose tolerance and a delayed insulin peak; both of these abnormalities were reversed by refeeding. These results suggest that although the guinea pig possesses an unusually high blood level of immunoreactive insulin, its insulin and glucose responses to fasting and glucose tolerance tests are similar to those reported for other species.