Relationship between the distribution of Phox2b expressing cells and blood vessels in the parafacial region of the ventral medulla of neonatal rats
Relationship between the distribution of Phox2b expressing cells and blood vessels in the parafacial region of the ventral medulla of neonatal rats
复制标题
新生大鼠腹延髓面旁区Phox2b表达细胞分布与血管的关系
DOI：
10.1016/j.neuroscience.2012.03.037
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发表时间：
2012
期刊：
影响因子：
3.3
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Onimaru;H.;Ikeda;K.;Kawakami;K.
据报道，中央化学感受器细胞在髓质中分布在紧密的贴壁毛细血管。在后斜方核（RTN）的Phox 2b表达神经元响应高CO2/H+刺激，并已被建议在中央化学感受中发挥重要作用。在新生大鼠中，RTN重叠至少部分与面旁呼吸组（pFRG），其中主要由吸气前神经元。在本研究中，我们可视化的血管在腹侧延髓的新生大鼠使用神经生物素的方法，并检查血管和Phox 2b免疫反应（IR）神经元的位置之间的关系。我们发现，Phox 2b-ir神经元在parfacial区域的头端腹侧延髓往往聚集在毛细血管周围。我们还证实，pFRG/吸气前神经元，在河豚毒素的存在下，高碳酸刺激敏感的Phox 2b-免疫反应神经元，并紧密贴附于血管沿着纵轴。我们的研究结果表明，Phox 2b-ir神经元的位置，包括吸气前神经元的pFRG，匹配其作为传感器的血液CO2浓度的作用。
It has been reported that central chemoreceptor cells in the medulla are distributed in close apposition to capillary blood vessels in the medulla. Phox2b-expressing neurons in the retrotrapezoid nucleus (RTN) respond to high CO2/H+stimulation and have been suggested to play an important role in central chemoreception. In newborn rats, the RTN overlaps at least partially with the parafacial respiratory group (pFRG), which consists predominantly of preinspiratory neurons. In the present study, we visualized the blood vessels in the ventral medulla of newborn rats using a neurobiotin method and examined the relationship between the blood vessels and the location of Phox2b-immunoreactive (-ir) neurons. We showed that Phox2b-ir neurons in the parafacial region of the rostral ventral medulla tended to assemble around capillary blood vessels. We also confirmed that pFRG/preinspiratory neurons that were sensitive to hypercapnic stimulation in the presence of tetrodotoxin were Phox2b-ir neurons and were tightly apposed to the blood vessels along the longitudinal axis. Our findings suggested that the location of Phox2b-ir neurons, including preinspiratory neurons of the pFRG, matched their role as sensors of blood CO2concentration.