Glial reaction to photoelectric dye-based retinal prostheses implanted in the subretinal space of rats
Glial reaction to photoelectric dye-based retinal prostheses implanted in the subretinal space of rats
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DOI：
10.1007/s10047-007-0398-8
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发表时间：
2008-04-01
影响因子：
1.3
通讯作者：
Ohtsuki, Hiroshi
中科院分区：
文献类型：
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作者：
Tamaki, Takayuki;Matsuo, Toshihiko;Ohtsuki, Hiroshi
我们设计了一种新型的视网膜假体，使用聚乙烯薄膜与光电染料分子结合，吸收光并将光子能量转换为电势。高密度聚乙烯的挤出吹塑膜用作原始聚乙烯膜。再结晶膜是通过从原始聚乙烯膜的熔融中再结晶而制成的。通过酰胺键将光电染料2-[2-[4-（二丁基氨基）苯基]乙烯基]-3-羧甲基溴化苯并噻唑偶联到两种类型的聚乙烯膜上。将原始染料偶联膜、染料偶联再结晶膜和染料未偶联平膜的样品植入正常成年大鼠的视网膜下腔。从1周或1个月时摘除的眼睛切下冷冻切片，并用苏木精和伊红染色，化学染色用于胶质细胞酸性蛋白（GFAP），或处理用于原位凋亡检测。结果显示，视网膜组织损伤可忽略不计，无炎性细胞和少量凋亡细胞。GFAP在植入不同类型聚乙烯膜后1周的视网膜部位较邻近视网膜部位显著上调（P < 0.005，方差分析）。在1个月时，平片和染料偶联再结晶片的GFAP表达也明显上调（P < 0.05）。1周和1个月之间，膜周围的胶质细胞包围显著增加（P = 0.023，双因素方差分析），但在三种类型的聚乙烯膜之间没有显著差异（P = 0.4531）。这些结果表明植入大鼠视网膜下腔的光电染料偶联聚乙烯膜具有神经胶质反应的证据，也证明了其基本的生物安全性。
We have designed a new type of retinal prosthesis using polyethylene films coupled with photoelectric dye molecules that absorb light and convert photon energy to electric potentials. An extruded-blown film of high-density polyethylene was used as the original polyethylene film. Recrystallized film was made by recrystallization from the melting of the original polyethylene film. A photoelectric dye,2-[2-[4-(dibutylamino)phenyl]ethenyl]-3-carboxymethylbenzothiazolium bromide, was coupled to the two types of polyethylene films through amide linkages. Samples of the original dye-coupled film, the dye-coupled recrystallized film, and the dye-uncoupled plain film were implanted in the subretinal space of normal adult rats. Frozen sections were cut from the eyes enucleated at 1 week or 1 month and were either stained with hematoxylin and eosin, stained immunohistochemically for glial fibrillary acidic protein (GFAP), or processed for in situ apoptosis detection. The results revealed that retinal tissue damage was negligible with no inflammatory cells and few apoptotic cells. GFAP was significantly up-regulated in retinal sites with the implantation of all types of polyethylene films at 1 week, compared with the adjacent retinal sites (P < 0.005, analysis of variance). The GFAP up-regulation was also present at 1 month for the plain film and dye-coupled recrystallized film (P < 0.05). Glial cell encirclement around the films increased significantly between 1 week and 1 month (P = 0.023, two-factor analysis of variance) but was not significantly different among the three types of polyethylene films (P = 0.4531). These results showed evidence of glial reactions to the photoelectric dye-coupled polyethylene films implanted into the subretinal space of rat eyes and also proved their basic biological safety.