Transcription Factor OsTGA10 Is a Target of the MADS Protein OsMADS8 and Is Required for Tapetum Development
Transcription Factor OsTGA10 Is a Target of the MADS Protein OsMADS8 and Is Required for Tapetum Development
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DOI：
10.1104/pp.17.01419
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发表时间：
2018-01-01
期刊：
影响因子：
7.4
通讯作者：
Bai, Shu-Nong
中科院分区：
文献类型：
--
作者：
Chen, Zhi-Shan;Liu, Xiao-Feng;Bai, Shu-Nong
这项研究旨在阐明花器官特性确定和组织发育背后的调控成分。目前尚不清楚器官识别蛋白如何促进器官原基发育成具有确定身份的组织，尽管根据ABC模型，花器官识别长期以来一直被认为是由识别基因的相互作用决定的。利用染色质免疫沉淀测序技术，我们确定了编码bZIP转录因子的OsTGA10是MADS盒蛋白OsMADS8的靶标，该蛋白被注释为E类器官识别蛋白。我们通过遗传和分子分析鉴定了OsTGA10的功能。OsTGA10在雄蕊发育过程中优先表达，OsTGA10突变导致雄性不育。OsTGA10是绒毡层发育所必需的，通过与已知的绒毡层基因相互作用而发挥作用。此外，在Ostga10雄蕊中，药室内壁的标志细胞壁增厚是有缺陷的。我们的研究结果表明，OsTGA10在水稻雄蕊发育的器官决定和绒毡层发育之间、绒毡层发育和小孢子发育之间以及绒毡层发育所需的各种调控成分之间起着中介作用。此外，Ostga10中的药室内壁缺陷意味着药室内壁的增厚依赖于绒毡层的发育。
This study aimed at elucidating regulatory components behind floral organ identity determination and tissue development. It remains unclear how organ identity proteins facilitate development of organ primordia into tissues with a determined identity, even though it has long been accepted that floral organ identity is genetically determined by interaction of identity genes according to the ABC model. Using the chromatin immunoprecipitation sequencing technique, we identified OsTGA10, encoding a bZIP transcription factor, as a target of the MADS box protein OsMADS8, which is annotated as an E-class organ identity protein. We characterized the function of OsTGA10 using genetic and molecular analyses. OsTGA10 was preferentially expressed during stamen development, and mutation of OsTGA10 resulted in male sterility. OsTGA10 was required for tapetum development and functioned by interacting with known tapetum genes. In addition, in ostga10 stamens, the hallmark cell wall thickening of the endothecium was defective. Our findings suggest that OsTGA10 plays a mediator role between organ identity determination and tapetum development in rice stamen development, between tapetum development and microspore development, and between various regulatory components required for tapetum development. Furthermore, the defective endothecium in ostga10 implies that cell wall thickening of endothecium is dependent on tapetum development.