The prevalence of enteric RNA viruses in stools from diarrheic and non-diarrheic people in southwestern Alberta, Canada
The prevalence of enteric RNA viruses in stools from diarrheic and non-diarrheic people in southwestern Alberta, Canada
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DOI：
10.1007/s00705-016-3063-9
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发表时间：
2017-01-01
影响因子：
2.7
通讯作者：
Houde, Alain
中科院分区：
文献类型：
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作者：
Leblanc, Danielle;Inglis, G. Douglas;Houde, Alain
阿尔伯塔西南部是加拿大的一个地区，肠炎发病率高，牲畜密度高。在2008年和2009年的1年期间，在来自腹泻（n = 2281）和非腹泻（n = 173）人群的粪便中评价了肠道RNA病毒，特别是诺如病毒（NoV）GI、GII、GIII、GIV;沙波病毒（SaV）;轮状病毒（RV）和星状病毒（AstV）的存在。腹泻个体生活在农村（46.6%）和城市（53.4%）环境中，年龄从不到1个月到102岁不等，这些个体中感染率最高的是11月。总共有388人（17.0%）的粪便中检出病毒. NoV GII是最常检出的病毒（8.0%; n = 182），其次是SaV（4.3%; n = 97）、RV（2.0%; n = 46）、AstV（1.8%; n = 42）、NoV GI（0.9%; n = 20）和NoV GIV（0.1%; n = 1）。从未检出动物NoV GIII。混合病毒感染的流行率在腹泻个体中为2.8%（n = 11）。1 ~ 5岁儿童粪便阳性率最高，其次是老年人（>= 70岁）。在非腹泻人群的粪便中仅检测到NoV GII（1.2%; n = 2）和SaV（1.2%; n = 2）。粪便的一个子集的序列分析显示同源性的NoV，SaV和RV序列从人类，但不是从非人类动物的菌株。本研究的结果不支持动物源性病毒对生活在阿尔伯塔西南部的人群中RNA肠道病毒引起的急性胃肠炎的发生有显著影响的假设。
Southwestern Alberta is a region of Canada that has high rates of enteritis as well as high densities of livestock. The presence of enteric RNA viruses, specifically norovirus (NoV) GI, GII, GIII, GIV; sapovirus (SaV); rotavirus (RV); and astrovirus (AstV), was evaluated in stools from diarrheic (n = 2281) and non-diarrheic (n = 173) people over a 1-year period in 2008 and 2009. Diarrheic individuals lived in rural (46.6 %) and urban (53.4 %) settings and ranged in age from less than 1 month to 102 years, and the highest prevalence of infection in these individuals was in November. In all, viruses were detected in diarrheic stools from 388 individuals (17.0 %). NoV GII was the most frequently detected virus (8.0 %; n = 182) followed by SaV (4.3 %; n = 97), RV (2.0 %; n = 46), AstV (1.8 %; n = 42), NoV GI (0.9 %; n = 20), and NoV GIV (0.1 %; n = 1). Animal NoV GIII was never detected. The prevalence of mixed viral infections in diarrheic individuals was 2.8 % (n = 11). Children from 1 to 5 years of age accounted for the highest prevalence of positive stools, followed by the elderly individuals (>= 70 years). Only NoV GII (1.2 %; n = 2) and SaV (1.2 %; n = 2) were detected in stools from non-diarrheic people. Sequence analysis of a subset of stools revealed homology to NoV, SaV and RV sequences from humans but not to strains from non-human animals. The results of this study do not support the hypothesis that viruses of animal origin have a significant impact on the occurrence of acute gastroenteritis caused by RNA enteric viruses in people living in southwestern Alberta.