Regulation of the Poly(A) Status of Mitochondrial mRNA by Poly(A)-Specific Ribonuclease Is Conserved among Land Plants
Regulation of the Poly(A) Status of Mitochondrial mRNA by Poly(A)-Specific Ribonuclease Is Conserved among Land Plants
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Poly(A) 特异性核糖核酸酶对线粒体 mRNA Poly(A) 状态的调节在陆地植物中是保守的
DOI：
10.1093/pcp/pcz212
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发表时间：
2019
影响因子：
4.9
通讯作者：
Hirayama Takashi
中科院分区：
文献类型：
--
作者：
Kanazawa Mai;Ikeda Yoko;Nishihama Ryuichi;Yamaoka Shohei;Lee Nam-Hee;Yamato Katsuyuki T;Kohchi Takayuki;Hirayama Takashi
在真核生物中，线粒体RNA的稳定性和质量的调节对于维持线粒体和细胞功能是必不可少的。我们以前曾报道过，AHG 2和AGS1分别编码的真核多聚腺苷酸特异性核糖核酸酶（PARN）和原核多聚腺苷酸聚合酶协调调节拟南芥线粒体mRNA的多聚腺苷酸状态和稳定性。PARN的线粒体功能在其他真核生物中尚未见报道。为了了解这种基于PARN的线粒体mRNA调控在植物中有多少是保守的，我们研究了地钱（Marchantia polymorpha）的AHG 2和AGS1对应物，地钱是基底陆地植物谱系的成员。我们发现M。AHG 2和AGS1各有一个直系同源物，分别命名为MpAHG 2和MpAGS1。在苔类植物的线粒体中检测到它们的柠檬酸融合蛋白。分子遗传学分析表明，MpAHG 2是必需的，并在功能上与MpAGS1相互作用，如在拟南芥中观察到的。重组MpAHG 2蛋白在体外具有去腺苷酶活性。MpAGS1的过表达和MpAHG 2的表达减少导致多聚腺苷酸化Mpcox 1mRNA的积累。此外，MpAHG 2抑制了hg2 - 1突变体的表型。这些结果表明，PARN为基础的线粒体mRNA调控系统是保守的陆地植物。
Regulation of the stability and the quality of mitochondrial RNA is essential for the maintenance of mitochondrial and cellular functions in eukaryotes. We have previously reported that the eukaryotic poly(A)-specific ribonuclease (PARN) and the prokaryotic poly(A) polymerase encoded byAHG2andAGS1, respectively, coordinately regulate the poly(A) status and the stability of mitochondrial mRNA in Arabidopsis. Mitochondrial function of PARN has not been reported in any other eukaryotes. To know how much this PARN-based mitochondrial mRNA regulation is conserved among plants, we studied the AHG2 and AGS1 counterparts of the liverwort,Marchantia polymorpha, a member of basal land plant lineage. We found thatM. polymorphahas one ortholog each forAHG2andAGS1, named MpAHG2and MpAGS1, respectively. Their Citrine-fused proteins were detected in mitochondria of the liverwort. Molecular genetic analysis showed that MpAHG2is essential and functionally interacts with MpAGS1as observed in Arabidopsis. A recombinant MpAHG2 protein had a deadenylase activity in vitro. Overexpression of MpAGS1and the reduced expression of MpAHG2caused an accumulation of polyadenylated Mpcox1mRNA. Furthermore, MpAHG2suppressed Arabidopsisahg2-1mutant phenotype. These results suggest that the PARN-based mitochondrial mRNA regulatory system is conserved in land plants.