Kinetics of mating in Physarum polycephalum.
Kinetics of mating in Physarum polycephalum.
复制标题
多头绒泡菌交配动力学。
DOI：
10.1080/00275514.1979.12020988
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发表时间：
1979
期刊：
影响因子：
2.8
通讯作者：
C. E. Holt
中科院分区：
文献类型：
--
作者：
D. Pallotta;P. Youngman;T. Shinnick;C. E. Holt
在含有相同数量的交配相容性变形虫的培养物中研究了通过交配形成的疟原虫。疟原虫形成之前总是有一段变形虫增殖期。当每次培养物的接种量从 3 X 103 到 1.6 X 10° 细胞总数变化时，当每次培养物的细胞总数达到约 5 X 105 时，疟原虫开始形成。当接种物中含有过量的第三种变形虫且无法与前两种变形虫交配时，当每个培养物的可交配细胞总数仅达到 1 x 10' 时，交配开始。第三种阿米巴原虫的这种效应并不是由它们食用用作食物的细菌引起的，因为直接限制食物细菌的数量并不会导致提前交配。当第三株变形虫通过成对的0.2克核孔过滤器与交配细胞分离时，过早交配仍然发生。因此，与无性疟原虫形成的情况一样，一种或多种细胞外因素似乎影响交配开始的时间。
Plasmodium formation by mating was studied in cultures containing equal numbers of mating-compatible amoebae. A period of amoebal multiplication always preceded plasmodium formation. When the inoculum varied from a total of 3 X 103 to 1.6 X 10° cells per culture, plasmodium formation commenced when the total number of cells reached about 5 X 105 per culture. When the inoculum included an excess of a third strain of amoebae that is unable to mate with the first two, mating commenced when the total number of matable cells reached only 1 x 10' per culture. This effect of the third strain of amoebae was not brought about by their consumption of the bacteria used as food, since directly limiting the number of food bacteria did not cause earlier mating. When amoebae of the third strain were separated from the mating cells by paired 0.2 gm Nuclepore filters, premature mating still occurred. Thus, as is the case with asexual plasmodium formation, one or more extracellular factors appear to influence the time of onset of mating.