Molecular design of heteroprotein assemblies providing a bionanocup as a chemical reactor
Molecular design of heteroprotein assemblies providing a bionanocup as a chemical reactor
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DOI：
10.1002/smll.200700855
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发表时间：
2008-01-01
期刊：
影响因子：
13.3
通讯作者：
Watanabe, Yoshihito
中科院分区：
文献类型：
--
作者：
Koshiyama, Tomomi;Yokoi, Norihiko;Watanabe, Yoshihito
含有合成分子的生物结构对于构建催化剂、传感器和具有新颖特性的材料来说是非常有吸引力的候选者。[1-11]蛋白质自组装特别适合这些目的，因为它们可以产生各种不同的纳米级笼结构。例如，已知病毒（例如豇豆褪绿斑驳病毒和烟草花叶病毒）、伴侣蛋白和铁蛋白等蛋白质组装体能够在其蛋白质笼中容纳金属纳米颗粒。[6,8,12,13]此外，还检查了荧光探针、金属复合物和药物在蛋白质表面上的共价结合。[7,9,14-17]虽然这些组装体对于各种功能的组织，很难控制附着在蛋白质表面的分子的数量和稳定性。我们研究了使用独特的笼子作为催化反应空间。该笼由异蛋白组装体组成，该组装体是从噬菌体 T4 中分离出来的基因产物 27 和基因产物 5 (gp27-gp5) 3 的三聚体（图 1）。 [18]噬菌体T4的蛋白质组装体
Bioarchitectures containing synthetic molecules are very attractive candidates for the construction of catalysts, sensors, and materials with novel properties.[1–11] Protein self-assemblies are especially suitable for these purposes because they can lead to various different nanoscale cage architectures. For example, protein assemblies such as viruses (eg, Cowpea chlorotic mottle virus and Tobacco mosaic virus), chaperonin, and ferritin are known to be able to accommodate metal nanoparticles in their protein cages.[6, 8, 12, 13] Additionally, the covalent conjugation of fluorescence probes, metal complexes, and drugs on protein surfaces has been examined.[7, 9, 14–17] While these assemblies are very useful for the organization of various functions, it is difficult to control the number and stability of the molecules attached on the protein surfaces.We have investigated the use of a unique cage as a catalytic reaction space. This cage consists of a heteroprotein assembly that is a trimer of gene product 27 and gene product 5,(gp27-gp5) 3, isolated from bacteriophage T4 (Figure 1).[18] This protein assembly of bacteriophageT4