Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
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发表时间：
1992-07
影响因子：
4
通讯作者：
S. Swasdison;Richard Mayne
中科院分区：
文献类型：
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作者：
S. Swasdison;Richard Mayne
开发了两种方法，其中建立了鹌鹑骨骼肌的长期培养物，使得所有的肌纤维以高度定向的方式发育。肌纤维自发地和有力地收缩，并在其末端与细胞外基质建立了牢固的连接，这些连接紧密地复制了肌腱连接的结构。在每根肌纤维周围形成一个连续的基底层，其中含有IV型胶原、层粘连蛋白和硫酸乙酰肝素蛋白聚糖。用其中一种方法，在每个肌纤维周围形成广泛的细胞外基质，其高度组织化，形成独特的肌外膜、肌束膜和肌内膜。通过两种方法对肌球蛋白不同亚型的培养物进行分析，结果表明一些肌细胞表达了肌球蛋白的成人形式。因此，结果表明，在本培养系统中，肌肉发育广泛进行了数周。现在可以直接研究肌纤维和细胞外基质之间的连接结构。
Two methods were developed in which long-term cultures of quail skeletal muscle were established so that all of the muscle fibers develop in a highly oriented manner. The muscle fibers became spontaneously and vigorously contractile and established strong connections with the extracellular matrix at their ends that closely duplicate the structure of the myotendinous junction. A continuous basal lamina was formed around each muscle fiber that contained type IV collagen, laminin and heparan sulfate proteoglycan. With one of the methods, an extensive extracellular matrix developed around each muscle fiber that was highly organized with the formation of a distinctive epimysium, perimysium and endomysium. Analysis of the cultures by both methods for different isoforms of myosin showed expression of an adult form of myosin by some of the muscle cells. The results therefore demonstrate that muscle development in the present culture systems proceeds extensively for several weeks. It will now be possible to investigate directly the structure of the connections between muscle fibers and the extracellular matrix.