Micro-morphological properties of osteons reveal changes in cortical bone stability during aging, osteoporosis, and bisphosphonate treatment in women
Micro-morphological properties of osteons reveal changes in cortical bone stability during aging, osteoporosis, and bisphosphonate treatment in women
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DOI：
10.1007/s00198-013-2374-x
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发表时间：
2013-10-01
影响因子：
4
通讯作者：
Busse, B.
中科院分区：
文献类型：
--
作者：
Bernhard, A.;Milovanovic, P.;Busse, B.
我们分析了年轻、健康老年人、未治疗骨质疏松症和双膦酸盐治疗骨质疏松症妇女的骨形态特征以及个体骨力学稳定性的几何指标。我们的研究揭示了组间骨形态和骨细胞腔隙的显著差异，表明不同的重塑模式与骨折易感性有关。骨重塑是骨结构重组的关键过程，其改变会导致骨力学强度的变化。由于骨反映了不同的骨重塑模式，我们假设不同年龄、疾病和治疗条件下的女性股骨皮质会表现出不同的骨形态和骨细胞腔隙数量以及不同的力学特性。方法在尸检中采集35例女性供体标本（年轻组n=6，年龄32 +/- 8岁；老年组n=10，年龄79 +/- 9岁；骨质疏松组n=10，年龄81 +/- 9岁；双磷酸盐组n=9，年龄81 +/- 7岁）。Von kossa染色股骨近端骨干切片评估骨形态参数和骨细胞腔隙数据。计算骨横截面的几何指数以评估单个骨的力学稳定性，包括其抗压缩、弯曲和屈曲的能力。结果年轻组、老年组、骨质疏松组和双膦酸盐治疗组的骨形态及骨细胞腔隙定量均有显著差异。计算的骨几何指数提供了基于其大小的单个骨的抗压缩，弯曲和屈曲的估计。特别是，二膦酸盐治疗组的骨呈现出改善的骨几何形状，并且骨细胞腔隙数量增加，这些骨细胞腔隙以前因衰老和骨质疏松而受损。结论骨（作为皮质骨的基本结构单位）在不同骨骼状况下的数据可用于突出影响不同人群骨折易感性的结构因素。
We analyzed morphological characteristics of osteons along with the geometrical indices of individual osteonal mechanical stability in young, healthy aged, untreated osteoporotic, and bisphosphonate-treated osteoporotic women. Our study revealed significant intergroup differences in osteonal morphology and osteocyte lacunae indicating different remodeling patterns with implications for fracture susceptibility.Introduction Bone remodeling is the key process in bone structural reorganization, and its alterations lead to changes in bone mechanical strength. Since osteons reflect different bone remodeling patterns, we hypothesize that the femoral cortices of females under miscellaneous age, disease and treatment conditions will display distinct osteonal morphology and osteocyte lacunar numbers along with different mechanical properties.Methods The specimens used in this study were collected at autopsy from 35 female donors (young group, n=6, age 32 +/- 8 years; aged group, n=10, age 79 +/- 9 years; osteoporosis group, n=10, age 81 +/- 9 years; and bisphosphonate group, n=9, age 81 +/- 7 years). Von Kossa-modified stained femoral proximal diaphyseal sections were evaluated for osteonal morphometric parameters and osteocyte lacunar data. Geometrical indices of osteonal cross-sections were calculated to assess the mechanical stability of individual osteons, in terms of their resistance to compression, bending, and buckling.Results The morphological assessment of osteons and quantification of their osteocyte lacunae revealed significant differences between the young, aged, osteoporosis and bisphosphonate-treated groups. Calculated osteonal geometric indices provided estimates of the individual osteons' resistance to compression, bending and buckling based on their size. In particular, the osteons in the bisphosphonate-treated group presented improved osteonal geometry along with increased numbers of osteocyte lacunae that had been formerly impaired due to aging and osteoporosis.Conclusions The data derived from osteons (as the basic structural units of the cortical bone) in different skeletal conditions can be employed to highlight structural factors contributing to the fracture susceptibility of various groups of individuals.