Probing strigolactone receptors in Striga hermonthica with fluorescence
Probing strigolactone receptors in Striga hermonthica with fluorescence
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DOI：
10.1126/science.aab3831
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发表时间：
2015-08-21
期刊：
影响因子：
56.9
通讯作者：
Hagihara, Shinya
中科院分区：
文献类型：
--
作者：
Tsuchiya, Yuichiro;Yoshimura, Masahiko;Hagihara, Shinya
阐明独脚金内酯的信号机制是控制寄生植物独脚金内酯危害的关键。为了克服先前干扰孤孤内酯受体鉴定的遗传顽固性，我们开发了一种荧光开启探针，Yoshimulactone Green (YLG)，它激活孤孤内酯信号传导并照亮孤孤内酯受体的信号感知。在这里，我们描述了独角独内酯如何结合并通过shhtl起作用，shhtl是Striga中分化的α / β水解酶折叠蛋白家族。利用YLGs进行的实时成像显示，孤角藻内酯感知的动态波状传播唤醒了孤角藻种子。我们认为ShHTLs作为独脚金内酯受体在独脚金内酯种子萌发中起调节作用。我们的发现使我们能够接触到孤孤内酯受体，并观察孤孤内酯信号转导的调控动力学。
Elucidating the signaling mechanism of strigolactones has been the key to controlling the devastating problem caused by the parasitic plant Striga hermonthica. To overcome the genetic intractability that has previously interfered with identification of the strigolactone receptor, we developed a fluorescence turn-on probe, Yoshimulactone Green (YLG), which activates strigolactone signaling and illuminates signal perception by the strigolactone receptors. Here we describe how strigolactones bind to and act via ShHTLs, the diverged family of alpha/beta hydrolase-fold proteins in Striga. Live imaging using YLGs revealed that a dynamic wavelike propagation of strigolactone perception wakes up Striga seeds. We conclude that ShHTLs function as the strigolactone receptors mediating seed germination in Striga. Our findings enable access to strigolactone receptors and observation of the regulatory dynamics for strigolactone signal transduction in Striga.