Microbial Adhesion on Different Bracket Types in vitro
Microbial Adhesion on Different Bracket Types in vitro
复制标题
DOI：
10.2319/092908-507.1
复制
发表时间：
2009-09-01
影响因子：
3.4
通讯作者：
Carels, Carine
中科院分区：
文献类型：
--
作者：
van Gastel, Jan;Quirynen, Marc;Carels, Carine
目的：验证7种不同支架类型在细菌总数和生物膜容量形成方面存在差异的假设。材料与方法：通过离体实验，比较了七种市售支架系统（Damon [A]、Clarity [B]、Mystique [C]、Speed [D]、Victory MBT [E]、Micro-loc [F]、Generus [G]）。每个托槽系统各25个前磨牙托槽在含2名正畸患者唾液和细菌的脑心灌注培养基中孵育。72h后，通过计数菌落形成单位（CFU）来测定需氧菌和厌氧菌的数量。计算好氧/厌氧菌CFU比，并对黑色色素菌进行分析。结果：不同托槽类型在生物膜形成方面存在显著差异。托架类型可任意分为低、中、高三种牙菌斑保持托架。低粘附组包括E型、F型和G型支架；A、B、C型支架高附着力组；D型为中等粘附性。高微生物黏附组（A、B和C）的CFU比率（需氧/厌氧）显著低于其他支架系统（P < 0.05）。结论：假设被接受。正畸托槽作为生物膜形成的不同位点；在这项体外研究中，不同类型的托槽之间存在显著差异。（直角正交，2009;79:915-921）
Objective: To test the hypothesis that there are differences in total bacterial counts and capacity biofilm formation between seven different bracket types. Material andMethods: By means of an in vitro experiment, seven commercially available bracket systems (Damon [A], Clarity [B], Mystique [C], Speed [D], Victory MBT [E], Micro-loc [F], and Generus [G]) were compared. A total of 25 premolar brackets of each bracket system were incubated in brain heart infusion medium containing the saliva and bacteria of two orthodontic patients. After 72 hours, the amounts of aerobe and anaerobe bacteria were determined by counting the colony-forming units (CFU). The CFU ratio (aerobe/anaerobe) also was calculated, and the black pigmented bacteria were analyzed.Results: Significant differences between the different bracket types in terms of biofilm formation were found. Bracket types can be arbitrarily divided into low, intermediate, and high plaque-retaining brackets. The group with low adhesion consists of bracket types E, F, and G; the group with high adhesion of bracket types A, B, and C; and type D exhibits intermediate adhesion. The group with high microbial adhesion (A, B, and C) did present significantly lower CFU ratios (aerobe/anaerobe) than were exhibited by the other bracket systems (P < .05).Conclusion: The hypothesis is accepted. Orthodontic brackets serve as different loci for biofilm formation; in this in vitro study, significant differences were noted between the different types of brackets. (Angle Orthod. 2009;79:915-921.)