Cutting edge:: Tlr5-/- mice are more susceptible to Escherichia coli urinary tract infection
Cutting edge:: Tlr5-/- mice are more susceptible to Escherichia coli urinary tract infection
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DOI：
10.4049/jimmunol.178.8.4717
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发表时间：
2007-04-15
影响因子：
4.4
通讯作者：
Aderem, Alan
中科院分区：
文献类型：
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作者：
Andersen-Nissen, Erica;Hawn, Thomas R.;Aderem, Alan
尽管TLR 5调节对细菌鞭毛蛋白的先天性免疫应答，但尚不清楚其功能在体内鼠感染期间是否是必需的。为了验证这个问题，我们用大肠杆菌经尿道攻击Tlr 5（-/-）小鼠。在感染后2天，与Tlr 5（-/-）小鼠相比，野生型小鼠表现出膀胱炎症增加。到感染后第5天，与野生型小鼠相比，Tlr 5（-/-）小鼠膀胱和肾脏中的细菌明显更多，并且两个器官中的炎症增加。此外，鞭毛蛋白诱导高水平的细胞因子和趋化因子表达的膀胱是依赖于TLR 5。总之，这些数据代表了TLR 5调节泌尿道中的先天性免疫应答的第一个证据，并且对于针对细胞外病原体的有效鼠体内免疫应答是必需的。
Although TLR5 regulates the innate immune response to bacterial flagellin, it is unclear whether its function is essential during in vivo murine infections. To examine this question, we challenged Tlr5(-/-) mice transurethrally with Escherichia coli. At 2 days postinfection, wild-type mice exhibited increased inflammation of the bladder in comparison to Tlr5(-/-) mice. By day 5 postinfection, Tlr5(-/-) mice had significantly more bacteria in the bladders and kidneys in comparison to wild-type mice and showed increased inflammation in both organs. In addition, flagellin induced high levels of cytokine and chemokine expression in the bladder that was dependent on TLR5. Together, these data represent the first evidence that TLR5 regulates the innate immune response in the urinary tract and is essential for an effective murine in vivo immune response to an extracellular pathogen.