Unilateral nasal obstruction induces degeneration of fungiform and circumvallate papillae in rats
Unilateral nasal obstruction induces degeneration of fungiform and circumvallate papillae in rats
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DOI：
10.1016/j.jfma.2017.04.013
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发表时间：
2018-03-01
影响因子：
3.2
通讯作者：
Ono, Takashi
中科院分区：
文献类型：
--
作者：
Hsu, Jui-Chin;Watari, Ippei;Ono, Takashi
背景：在临床正畸治疗中，慢性呼吸障碍或口呼吸一直是备受关注的症状和筛查标准。本研究通过建立单侧鼻塞大鼠模型，观察大鼠味觉乳头和味蕾的变化，探讨鼻塞与味觉感受的关系。方法：将14只6日龄雄性Wistar大鼠随机分为对照组和实验组，每组7只。实验组在8日龄时行单侧鼻塞术。在9周龄时对大鼠实施安乐死。用免疫组织化学方法观察轮廓乳头和味蕾的形态。结果：单侧鼻腔阻塞后，味觉上皮细胞出现缺损，味觉细胞的形态学改变不明显。实验组大鼠的菌状乳头和味蕾体积明显减少。结论：鼻阻塞可能通过影响舌区味蕾和味觉乳头的形态学改变而影响味觉功能。版权所有（C）2017，台湾医学会.出版社：Elsevier Taiwan LLC
Background: In clinical orthodontic treatment, chronic respiratory disturbance or mouth breathing has been concerned symptoms and screening criteria. In this study, to analyze the relation between nasal obstruction and taste sensing, a unilateral nasal obstruction model was performed to investigate the taste papillae and taste buds in rats.Methods: Fourteen 6-day-old male Wistar rats were randomly divided into control and experimental groups (n = 7 each). The experimental group underwent unilateral nasal obstruction at 8 days of age. The rats were euthanized at 9-week-old. The morphology of the circumvallate papillae and taste buds were identified by immunohistochemical methods. The fungiform papillae were visualized with 1% methylene blue and sectioned for taste bud observation.Results: Some defects in the gustatory epithelium were observed after unilateral nasal obstruction. Rats in the experimental group had significantly fewer fungiform papillae and smaller volumes of taste bud. In circumvallate papillae, smaller total taste bud area was found in experiment group.Conclusion: Findings in the present study suggest that nasal obstruction might have significant influences on the gustatory function via morphologic change in the taste papillae and taste buds in tongue area. Copyright (C) 2017, Formosan Medical Association. Published by Elsevier Taiwan LLC.