Dual-color oligo-FISH can reveal chromosomal variations and evolution in Oryza species
Dual-color oligo-FISH can reveal chromosomal variations and evolution in Oryza species
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双色寡核苷酸 FISH 可以揭示水稻物种的染色体变异和进化
DOI：
10.1111/tpj.14522
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发表时间：
2019-10-12
期刊：
影响因子：
7.2
通讯作者：
Gong, Zhiyun
中科院分区：
文献类型：
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作者：
Liu, Xiaoyu;Sun, Shang;Gong, Zhiyun
基于寡核苷酸探针的荧光原位杂交是一种用于染色体鉴定和核型分析的有用工具。在这里，我们开发了两个寡核苷酸FISH探针，可以识别水稻(Oryza Sativa)12对染色体中的每一对。这两个探针分别由25个717(绿色)和25 215(红色)寡核苷酸(45个核苷酸)组成，并产生了26个不同的FISH信号，可以用作条形码来唯一标记12对水稻染色体中的每一对。利用该系统建立了水稻有丝分裂染色体和减数分裂染色体的标准核型。此外，还利用双色寡核苷酸杂交技术对不同类型的染色体异常进行了分析。使用这些探针在不同野生稻物种中进行的寡聚荧光原位杂交分析表明，来自AA、BB或CC基因组的染色体产生了与水稻相似的特异而强烈的信号，而带有EE基因组的染色体产生的特异信号较少，而FF基因组则没有信号。总之，我们建立的寡头FISH探针将成为研究水稻属染色体变异和进化的有力工具。
Fluorescence in situ hybridization using probes based on oligonucleotides (oligo-FISH) is a useful tool for chromosome identification and karyotype analysis. Here we developed two oligo-FISH probes that allow the identification of each of the 12 pairs of chromosomes in rice (Oryza sativa). These two probes comprised 25 717 (green) and 25 215 (red) oligos (45 nucleotides), respectively, and generated 26 distinct FISH signals that can be used as a barcode to uniquely label each of the 12 pairs of rice chromosomes. Standard karyotypes of rice were established using this system on both mitotic and meiotic chromosomes. Moreover, dual-color oligo-FISH was used to characterize diverse chromosomal abnormalities. Oligo-FISH analyses using these probes in various wild Oryza species revealed that chromosomes from the AA, BB or CC genomes generated specific and intense signals similar to those in rice, while chromosomes with the EE genome generated less specific signals and the FF genome gave no signal. Together, the oligo-FISH probes we established will be a powerful tool for studying chromosome variations and evolution in the genus Oryza.