Effects of Traditional Kampo Drugs and Their Constituent Crude Drugs on Influenza Virus Replication In Vitro: Suppression of Viral Protein Synthesis by Glycyrrhizae Radix
Effects of Traditional Kampo Drugs and Their Constituent Crude Drugs on Influenza Virus Replication In Vitro: Suppression of Viral Protein Synthesis by Glycyrrhizae Radix
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DOI：
10.1155/2019/3230906
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发表时间：
2019-12-03
影响因子：
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通讯作者：
Sakaguchi, Takemasa
中科院分区：
文献类型：
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作者：
Nomura, Toshihito;Fukushi, Masaya;Sakaguchi, Takemasa
流感病毒流行是公共卫生中的重要问题，需要持续开发有效的药物。汉方医学是一种传统医学，在日本和其他东亚国家临床上用于治疗各种疾病。我们评估了汉方药物maoto、kakkonto、senkyuchachosan、jinkokato和bakumondoto对培养细胞中流感病毒复制的影响，这些汉方药物用于治疗包括流感引起的症状在内的呼吸道症状。在加入不同浓度的汉方药物后24 h，检测流感病毒感染MDCK（+）细胞的培养基的血凝和感染性，发现5种汉方药物中有4种抑制病毒释放到培养基中。这些药物不是通过作用于病毒颗粒而是通过作用于病毒感染的细胞来灭活病毒的感染性。此外，当检查构成有效汉方药物的六种生药（苍术、川芎、蛇床子、甘草、黄芪和黄芪）时，发现选择性抑制病毒蛋白合成的黄芪活性最强（IC 50 = 0.27 mg/ml）。由于在许多汉方药物中含有黄芪，因此它可能会为广泛的汉方药物提供抗流感病毒活性。
An influenza virus epidemic is an important issue in public hygiene, and continuous development on an effective drug is required. Kampo medicine is a traditional medicine that is used clinically for treatment of various diseases in Japan and other East Asian countries. We evaluated the effects of the Kampo drugs maoto, kakkonto, senkyuchachosan, jinkokato, and bakumondoto, which are prescribed for treatment of respiratory symptoms including symptoms caused by influenza, on influenza virus replication in cultured cells. Culture media of influenza virus-infected MDCK(+) cells were tested for hemagglutination and infectivity at 24 h after the addition of Kampo drugs at various concentrations, and four of the five Kampo drugs were found to inhibit virus release to the culture media. These drugs inactivated virus infectivity not by acting on virus particles but by acting on virus-infected cells. In addition, when six crude drugs (Atractylodis lanceae rhizome, Citri unshiu pericarpium, Cnidii rhizome, Glycyrrhizae radix, Rehmanniae radix, and Saposhnikoviae radix) that constitute the effective Kampo drugs were examined, the strongest activity was found for Glycyrrhizae radix (IC50 = 0.27 mg/ml), which selectively suppressed viral protein synthesis. Since Glycyrrhizae radix is contained in many Kampo drugs, it may give anti-influenza virus activity to a broad range of Kampo drugs.