Renal adaptation to changes in dietary phosphate during development.
Renal adaptation to changes in dietary phosphate during development.
复制标题
发育过程中肾脏对膳食磷酸盐变化的适应。
DOI：
10.1152/ajprenal.1990.258.6.f1650
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Haramati,A
中科院分区：
文献类型：
--
作者：
Mulroney,SE;Haramati,A
本研究验证了这一假设，即膳食磷酸盐（Pi）对肾脏处理Pi的影响在发育过程中发生变化。我们评估了饮食中Pi含量的变化是否会改变未成年大鼠中Pi重吸收的肾小管能力[最大RPi/肾小球滤过率（GFR）]，与成年大鼠相比，未成年大鼠的最大RPi/GFR相对较大。然后，我们研究了未成年和成年Pi剥夺大鼠的反应，膳食补充Pi。在三个不同发育阶段的急性甲状腺甲状旁腺切除Wistar大鼠中进行研究：未成熟（3至4周龄），年轻（6至7周龄）和成年（12至13周龄）。动物喂食低（0.07%）、正常（0.7%）或高（1.8%）磷酸盐饲料（分别为LPD、NPD和HPD）4天，然后准备进行肾清除率实验，以确定最大RPi/GFR。在所有饮食方案中，未成熟大鼠的最大RPi/GFR最高，并随着年龄的增长而逐渐下降。当喂食LPD时，磷酸盐转运速率已经升高的未成熟大鼠的最大RPi/GFR显著增加68 +/- 13%（从5.58 +/- 0.29增加到9.47 +/- 0.76 mumol/ml，P <0.01）。这显著大于在成年大鼠中观察到的38 +/- 3%增加（从3.50 +/- 0.18至4.81 +/- 0.09 mumol/ml）。相反，在对HPD的反应中，与年轻大鼠（-54 +/-3%）或成年大鼠（-61 +/-6%）的减少相比，未成熟大鼠（-42 +/-2%）的最大RPi/GFR降低最小。（250字处删节）
The present study tested the hypothesis that the influence of dietary phosphate (Pi) on the renal handling of Pi changes during development. We evaluated whether variations in the dietary Pi content would alter the tubular capacity of Pi reabsorption [Max RPi/glomerular filtration rate (GFR)] in immature rats, which have a relatively greater Max RPi/GFR compared with adult rats. Then we examined the response of immature and adult Pi-deprived rats to dietary Pi replenishment. Studies were performed in acutely thyroparathyroidectomized Wistar rats at three different stages of development: immature (3- to 4-wk old), young (6- to 7-wk old), and adult (12- to 13-wk old). Animals were fed either low (0.07%)-, normal (0.7%), or high (1.8%)-phosphate diet (LPD, NPD, and HPD, respectively) for 4 days and were then prepared for renal clearance experiments to determine the Max RPi/GFR. On all dietary regimens, the Max RPi/GFR was highest in immature rats and decreased progressively with age. When fed LPD, immature rats, with an already elevated rate of phosphate transport, displayed a remarkable 68 +/- 13% increase in the Max RPi/GFR (from 5.58 +/- 0.29 to 9.47 +/- 0.76 mumol/ml, P less than 0.01). This was significantly greater than the 38 +/- 3% increase observed in adult rats (from 3.50 +/- 0.18 to 4.81 +/- 0.09 mumol/ml). Conversely, in response to HPD, the decrease in the Max RPi/GFR was smallest in immature rats (-42 +/- 2%) compared with the decrement in either young (-54 +/- 3%) or adult (-61 +/- 6%) rats.(ABSTRACT TRUNCATED AT 250 WORDS)