Oligoclonal bands in cerebrospinal fluid: a comparative study of isoelectric focusing, agarose gel electrophoresis and IgG index
Oligoclonal bands in cerebrospinal fluid: a comparative study of isoelectric focusing, agarose gel electrophoresis and IgG index
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DOI：
10.1034/j.1600-0404.2000.102005322.x
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发表时间：
2000-11-01
影响因子：
3.5
通讯作者：
Vedeler, CA
中科院分区：
文献类型：
--
作者：
Lunding, J;Midgard, R;Vedeler, CA
目的：比较等电聚焦（IEF）、免疫固定、琼脂糖凝胶电泳（AGE）和IgG指数检测多发性硬化（MS）和其他神经系统疾病鞘内合成IgG的敏感性和特异性。材料和方法-脑脊液（CSF）和血清从147例患者的各种神经系统疾病，其中20人有MS，进行了比较IEF，AGE和IgG指数。结果-CSF限制性寡克隆带（OCB）中发现20 20 MS患者使用IEF和9 20使用AGE。12例其他神经系统疾病（OND）患者IEF检测到OCB，4例AGE检测到OCB。OND组平均IgG指数为0.50，MS组平均IgG指数为0.96（P <0.0001）。在20名MS患者中，9名患者的IgG指数高于规定的临界值0.72。结论IEF检测MS患者OCB的灵敏度约为AGE的2倍，IEF检测鞘内IgG合成的灵敏度也远优于IgG指数。
Objective - To compare the sensitivity and specificity of isoelectric focusing (IEF) with immunofixation, agarose gel electrophoresis (AGE) and the IgG index in detecting intrathecally synthesized IgG in multiple sclerosis (MS) and in other nervous system disorders. Materials and methods - Cerebrospinal fluid (CSF) and serum from 147 patients with various nervous system diseases, 20 of whom had MS, were compared with IEF, AGE and the IgG index. Results - CSF-restricted oligoclonal bands (OCB) were found in 20 of 20 patients with MS using IEF and in 9 of 20 using AGE. OCB were found in 12 patients with other nervous system disorders (OND) using IEF and 4 using AGE. The mean IgG index was 0.50 in OND and 0.96 in MS (P < 0.0001). Of 20 MS patients, 9 had an IgG index above the defined cut-off value of 0.72. Conclusions IEF is about twice as sensitive as AGE in detecting OCB in MS. IEF is also far superior to the IgG index in determining intrathecal IgG synthesis.