Measurements of CO2, lactic acid and sodium bicarbonate secreted by cultured cells using a flow-through type pH/CO2 sensor system based on ISFET
Measurements of CO2, lactic acid and sodium bicarbonate secreted by cultured cells using a flow-through type pH/CO2 sensor system based on ISFET
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DOI：
10.1016/j.snb.2005.10.029
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发表时间：
2006-05-23
影响因子：
8.4
通讯作者：
Kajiya, F
中科院分区：
文献类型：
--
作者：
Mohri, S;Shimizu, J;Kajiya, F
我们开发了一个pH/CO2传感器系统，使用两个离子敏感场效应晶体管（ISFET）来评估培养细胞的代谢活性。一个ISFET测量总pH值变化，另一个ISFET覆盖有透气硅管，测量CO2引起的pH值变化。将这些ISFET放置在具有窗口的流通室中，该窗口附接到在其上培养细胞的玻璃板上。用低缓冲容量的基线溶液进行测量。在细胞产物的定量估计中，我们假设细胞分泌CO2、乳酸和碳酸氢钠（NaHCO 3），然后乳酸和NaHCO 3进行中和反应，形成乳酸钠和额外的CO2。我们应用该系统来评估牛主动脉内皮细胞（BAEC）和兔主动脉平滑肌细胞（RASMC）的代谢活性。RASMC的CO2产量显著高于BAEC（分别为204 +/- 16和4.1 +/- 0.3 x 10（8）分子/细胞/秒）。在RASMC中产生的NaHCO 3多于乳酸，而在BAEC中它们没有显著差异。(c)2005 Elsevier B. V.保留所有权利。
We developed a pH/CO2 sensor System using two ion-sensitive field effect transistors (ISFETs) to evaluate the metabolic activity of cultured cells. One ISFET measures total pH changes and the other ISFET, covered with a gas-permeable silicon tube, measures pH changes due to CO2. These ISFETs were placed in a flow-through chamber with a window to be attached to a glass plate on which cells were cultured. Measurements were performed with a baseline solution of low buffer capacity. In the quantitative estimation of cellular products, we assumed that cells secreted CO2, lactic acid and sodium bicarbonate (NaHCO3), and then lactic acid and NaHCO3 proceeded to the neutralization reaction to form sodium lactate and additional CO2. We applied this system to evaluate the metabolic activity of bovine aortic endothelial cells (BAEC) and rabbit aortic smooth muscle cells (RASMC). The production of CO2 by RASMC was significantly higher than that by BAEC (204 +/- 16 and 4.1 +/- 0.3 x 10(8) molecules/cell/s, respectively). More NaHCO3 than lactic acid was produced in RASMC, whereas they were not significantly different in BAEC. (c) 2005 Elsevier B.V. All rights reserved.