Association of H-FABP gene polymorphisms with intramuscular fat content in Three-yellow chickens and Hetian-black chickens.
Association of H-FABP gene polymorphisms with intramuscular fat content in Three-yellow chickens and Hetian-black chickens.
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三黄鸡、和田乌鸡H-FABP基因多态性与肌内脂肪含量的相关性
DOI：
10.1186/s40104-016-0067-y
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发表时间：
2016
影响因子：
7
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Wang Y;Hui X;Wang H;Kurban T;Hang C;Chen Y;Xing J;Wang J
为探讨心肌型脂肪酸结合蛋白（heart-type fatty acid binding protein，H-FABP）基因与肌内脂肪（intramuscular fat，IMF）的关系，对60只三黄鸡（TYCs）和60只和田黑鸡（HTBCs）的H-FABP基因第二外显子进行了多态性分析。与TYC相比，HTBC的心肌、胸肌和腿肌IMF含量增加。根据χ2检验，TYC和HTBC群体均表现出Hardy-Weinberg平衡（HWE）。在这两种鸡中检测到3个变异，即G939 A、G982 A和C1014 T。与TC基因型相比，具有TT基因型的HTBC在胸肌中表现出增加的IMF含量。因此，G982 A位点可以被认为是选择增加的IMF含量在HTBC的胸肌的遗传标记。相关系数分析表明，HTBC组心肌、胸肌和腿肌中H-FABP mRNA表达与IMF含量呈负相关，TYC组心肌和胸肌中H-FABP mRNA表达与IMF含量呈负相关。与TYC相比，HTBC的心脏和腿部肌肉中H-FABP的相对mRNA表达降低，但在蛋白质水平上未观察到这种差异，通过Western blot分析评估。这些发现为HTBC的育种计划和未来研究H-FABP在鸡IMF沉积和调节中的作用提供了必要的数据。
To explore the relationship between the heart-type fatty acid binding protein (H-FABP) gene and intramuscular fat (IMF), a polymorphism of the second exon of the H-FABP gene was investigated in 60 Three-yellow chickens (TYCs) and 60 Hetian-black chickens (HTBCs). The IMF contents of the cardiac, chest and leg muscles in HTBC were increased compared with TYC. Both TYC and HTBC populations exhibited Hardy-Weinberg Equilibrium (HWE) according to the χ2 test. Three variations of the two birds were detected, namely, G939A, G982A and C1014T. HTBCs with the TT genotypes exhibit increased IMF content in the chest muscles compared with the TC genotype. Thus, the G982A site could be considered a genetic marker for selecting increased IMF content in the chest muscles of HTBC. The correlation coefficients revealed that H-FABP mRNA expression was negatively correlated with the IMF content in the cardiac, chest and leg muscles of HTBC and in the cardiac and chest muscles of TYC. The relative mRNA expression of H-FABP was reduced in the cardiac and leg muscles of HTBC compared with TYC, but this difference was not observed at the protein level, as assessed by Western blot analysis. These findings offer essential data that can be useful in the breeding program of HTBC and future research exploring the role of H-FABP in IMF deposition and regulation in chickens.