C-TYPE NATRIURETIC PEPTIDE - A SELECTIVE CARDIOVASCULAR PEPTIDE
C-TYPE NATRIURETIC PEPTIDE - A SELECTIVE CARDIOVASCULAR PEPTIDE
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DOI：
10.1152/ajpregu.1993.264.2.r290
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发表时间：
1993-02-01
影响因子：
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通讯作者：
BURNETT, JC
中科院分区：
文献类型：
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作者：
CLAVELL, AL;STINGO, AJ;BURNETT, JC
在三组麻醉狗中进行研究，比较结构相关的肽心房钠尿肽 (ANP) 和 C 型钠尿肽 (CNP)。第 1 组 (n = 5) 和第 2 组 (n = 4) 分别接受剂量为 10 ng 的 CNP 或 ANP 静脉输注。公斤-1。分钟 1 和 100 纳克。公斤-1。分钟-1。第 3 组 (n = 5) 肾内接受剂量为 1 ng 的 CNP。公斤-1。 min-1 和 5 ng-kg-1 。分钟-1。与 ANP 相比，静脉输注 CNP 导致血压下降幅度更大。使用 CNP 观察到的血压显着降低与 3',5'-环单磷酸鸟苷 (cGMP) 的显着较小增加相关。相比之下，CNP 的静脉内或肾内给药均与 ANP 观察到的尿钠排泄无关。目前的研究还证明了犬血浆中低皮摩尔浓度下存在 CNP 免疫反应性。凝胶渗透色谱的进一步表征表明，循环 CNP 免疫反应性对应于肽的 22 个氨基酸形式。总之，本研究表明 CNP 以低皮摩尔浓度循环，并且在体内具有强大的血管活性，表明其在调节血管张力方面具有潜在作用。
Studies were performed in three groups of anesthetized dogs to compare the structurally related peptides atrial natriuretic peptide (ANP) and C-type natriuretic peptide (CNP). Group 1 (n = 5) and group 2 (n = 4) received intravenous infusions of CNP or ANP respectively at doses of 10 ng . kg-1 . min-1 and 100 ng . kg-1 . min-1. Group 3 (n = 5) received CNP intrarenally at doses of 1 ng . kg-1 . min-1 and 5 ng-kg-1 . min-1. Intravenous infusion of CNP resulted in a greater decrease in blood pressure when compared with ANP. This marked decrease in blood pressure observed with CNP was associated with a significantly smaller increase in guanosine 3',5'-cyclic monophosphate (cGMP). In contrast, neither intravenous nor intrarenal administration of CNP was associated with natriuresis as observed with ANP. The current study also demonstrates the presence of CNP immunoreactivity in canine plasma at low picomolar concentrations. Further characterization by gel permeation chromatography demonstrated that circulating CNP immunoreactivity corresponds to the 22-amino acid form of the peptide. In conclusion, this study demonstrates that CNP circulates in low picomolar concentrations and is potently vasoactive in vivo, suggesting a potential role in the regulation of vascular tone.