Phenotypic and functional profiles of CRIg (Z39Ig)-expressing macrophages in the large intestine
Phenotypic and functional profiles of CRIg (Z39Ig)-expressing macrophages in the large intestine
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DOI：
10.1177/1753425911400641
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发表时间：
2012-04-01
期刊：
影响因子：
3.2
通讯作者：
Matsuyama, Takami
中科院分区：
文献类型：
--
作者：
Tanaka, Masashi;Nagai, Taku;Matsuyama, Takami
肠道巨噬细胞（mphi）通过有效清除大肠内的外来颗粒，在维持体内平衡中发挥重要作用。然而，功能性补体受体尚未完全确定。在这项研究中，我们发现Ig超家族的补体受体（CRIg，也称为Z39Ig），一种补体片段（C3b和iC3b）的受体，在小鼠和人类大肠的肠M phi的一个亚群上表达。当检测小鼠CRIg(+) M phi对抗原的摄取能力时，肠道CRIg(+) M phi表现出较低的内吞能力和与腹膜F4/80(+)CRIg(-) M phi和F4/80(+)CRIg(+) M phi相似的吞噬能力。此外，我们发现大肠杆菌c3b依赖性吞噬的很大一部分涉及CRIg，强调了有效机制消除大肠外来颗粒的重要性。另一方面，2,4,6-三硝基苯磺酸处理小鼠的肠道M phi降低了CRIg表达，但增加了CD11b表达，这可能对免疫复合物的清除有一定的贡献。这项研究将为大肠杆菌的调理和吞噬提供新的线索。
Intestinal macrophages (M phi) play significant roles in maintaining homeostasis by the efficient elimination of foreign particles in the large intestine. However, functional complement receptors have not been fully identified. In this study, we showed that a complement receptor of the Ig superfamily (CRIg, also known as Z39Ig), a receptor for complement fragments (C3b and iC3b), was expressed on a subset of intestinal M phi in murine and human large intestine. When abilities of uptake of antigens of murine CRIg(+) M phi were examined, intestinal CRIg(+) M phi displayed less endocytic and similar phagocytic abilities compared to resident peritoneal F4/80(+)CRIg(-) M phi and F4/80(+)CRIg(+) M phi. Additionally, we found that a significant portion of C3b-dependent phagocytosis by large intestinal M phi involves CRIg, emphasizing the importance of efficient mechanisms to eliminate foreign particles in the large intestine. On the other hand, intestinal M phi from 2,4,6-trinitrobenzene sulfonic acid-treated mice had decreased CRIg expression but increased CD11b expression, implying some contribution to the removal of immune complexes. This study will shed new light on opsonization and phagocytosis by large intestinal M phi.