Distribution of cytochrome c in polyacrylate microgels
Distribution of cytochrome c in polyacrylate microgels
复制标题
DOI：
10.1039/c0sm00072h
复制
发表时间：
2010-01-01
期刊：
影响因子：
3.4
通讯作者：
Hansson, Per
中科院分区：
文献类型：
--
作者：
Johansson, Christian;Hansson, Per
用显微操作器辅助光学显微镜和激光扫描共聚焦显微镜研究了弱交联聚丙烯酸钠微凝胶对水溶液(pH=7，离子强度40 mm)中细胞色素c的吸附。结果表明，在平衡状态下，蛋白质在凝胶中分布均匀。通过时间分辨的溶胀曲线和荧光标记的细胞色素c在单个凝胶颗粒中的扩散前沿的观察，研究了相互作用的动力学。凝胶达到溶胀平衡状态的时间通常很短，并且随着溶液中蛋白质浓度的增加而迅速减少，这表明微凝胶内的扩散很快。将结果与溶菌酶与相同类型网络结合的结果以及文献中关于大凝胶的结果进行了比较。强调了净吸引蛋白质-蛋白质作用力对结合机制的重要性。
The uptake of cytochrome c from aqueous solution (pH = 7, ionic strength 40 mM) into weakly crosslinked sodium polyacrylate microgels is investigated by means of micromanipulator-assisted light microscopy and laser scanning confocal microscopy. It is shown that the protein is distributed uniformly in the gels at equilibrium. The kinetics of the interaction is investigated by means of time-resolved deswelling curves, and by observations of the diffusion fronts of fluorescently labeled cytochrome c in single gel particles. The time to reach the deswollen equilibrium state of the gels is short, in general, and decreases rapidly with increasing protein concentration in the solution, indicative of a rapid diffusive transport inside the microgels. The results are compared with results for lysozyme binding with the same type of network, and with results for macrogels in the literature. The importance of net attractive protein-protein forces for the mechanism of binding is highlighted.