Genetic variation in NIN1 and C/VIF1 genes is significantly associated with Populus angustifolia resistance to a galling herbivore, Pemphigus betae.
Genetic variation in NIN1 and C/VIF1 genes is significantly associated with Populus angustifolia resistance to a galling herbivore, Pemphigus betae.
复制标题
NIN1 和 C/VIF1 基因的遗传变异与杨树对食草动物天疱疮的抗性显着相关。
DOI：
10.1016/j.jinsphys.2015.10.007
复制
发表时间：
2016
影响因子：
2.2
通讯作者：
Allan,GerardJ
中科院分区：
文献类型：
--
作者：
Zinkgraf,MatthewS;Meneses,Nashelly;Whitham,ThomasG;Allan,GerardJ
与生态重要性状相关的基因的鉴定提供了有关生物体对其自然环境反应的潜在遗传机制的信息。在这项研究中，我们在 154 个窄叶杨（Populus angustifolia）的自然种群中研究了宿主植物对诱导瘿蚜虫天疱疮（Pemphigus betae）的抗性的遗传基础。我们调查了两个被认为与库-源关系相关的基因和一个物候基因的遗传变异，该基因共位于先前确定的抗磨损数量性状基因座中。使用候选基因方法，出现了三个主要发现。首先，树木抗磨损的自然变化是可重复的。 1986 年初次调查 20 年后对同一树木基因型的采样表明，80% 的抗性变异是由于个体之间的遗传差异造成的。其次，我们发现植物中性蔗糖酶基因 NIN1 与树木抗性之间的单核苷酸多态性和单倍型水平存在显着关联。蔗糖酶是一类蔗糖水解酶，在植物对生物胁迫的反应中发挥着重要作用，包括建立营养库。这些与 NIN1 的关联是由位于该基因第二个内含子中的单核苷酸多态性 (NIN1_664) 驱动的，并且位于两个已知调控元件的直系同源序列中。第三，来自转化酶抑制剂（C/VIF1）的单倍型与树木抗性显着相关。这两个基因遗传变异的鉴定为了解有助于树木抵抗瘿形成的可能遗传机制提供了一个起点。我们还以先前的工作为基础，证明宿主库源关系的遗传差异会影响 P 的能力。 β-eto 操纵营养物质的流动并诱导营养物质汇。
The identification of genes associated with ecologically important traits provides information on the potential genetic mechanisms underlying the responses of an organism to its natural environment. In this study, we investigated the genetic basis of host plant resistance to the gall-inducing aphid,Pemphigus betae, in a natural population of 154 narrowleaf cottonwoods (Populus angustifolia). We surveyed genetic variation in two genes putatively involved in sink-source relations and a phenology gene that co-located in a previously identified quantitative trait locus for resistance to galling. Using a candidate gene approach, three major findings emerged. First, natural variation in tree resistance to galling was repeatable. Sampling of the same tree genotypes 20 years after the initial survey in 1986 show that 80% of the variation in resistance was due to genetic differences among individuals. Second, we identified significant associations at the single nucleotide polymorphism and haplotype levels between the plant neutral invertase geneNIN1and tree resistance. Invertases are a class of sucrose hydrolyzing enzymes and play an important role in plant responses to biotic stress, including the establishment of nutrient sinks. These associations withNIN1were driven by a single nucleotide polymorphism (NIN1_664) located in the second intron of the gene and in an orthologous sequence to two known regulatory elements. Third, haplotypes from an inhibitor of invertase (C/VIF1) were significantly associated with tree resistance. The identification of genetic variation in these two genes provides a starting point to understand the possible genetic mechanisms that contribute to tree resistance to gall formation. We also build on previous work demonstrating that genetic differences in sink-source relationships of the host influence the ability ofP. betaeto manipulate the flow of nutrients and induce a nutrient sink.