Expression of a new G protein-coupled receptor X-msr is associated with an endothelial lineage in Xenopus laevis
Expression of a new G protein-coupled receptor X-msr is associated with an endothelial lineage in Xenopus laevis
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DOI：
10.1016/0925-4773(96)00585-0
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发表时间：
1996-10-01
影响因子：
2.6
通讯作者：
Audigier, Y
中科院分区：
文献类型：
--
作者：
Devic, E;Paquereau, L;Audigier, Y
为了确定G蛋白偶联受体是否在早期胚胎发生中发挥作用，我们寻找位于跨膜片段的共有序列中的引物之间扩增的cDNA片段。使用一个这样的扩增片段作为探针，我们克隆了一个新的成员的G蛋白偶联受体超家族在非洲爪蟾。推导的蛋白质序列与其他受体的比对揭示了与血管紧张素受体的一些同源性。在囊胚晚期检测到2.5 kb的单个转录物，并且其表达在原肠胚形成期间增加。原位杂交显示转录本最初在腹外侧内卷边缘区，后来在侧板中胚层。在幼虫阶段，转录物在心前管和形成血管中表达，在那里它位于内内皮层中。因此，该基因追溯了内皮细胞谱系，并代表了爪蟾中心脏和血管内皮细胞特化的非常早期和独特的标记。我们建议将间充质相关蛇形受体命名为X-msr。
In order to determine whether G protein-coupled receptors play a role in early embryogenesis, we looked for cDNA fragments amplified between primers located in consensus sequences of transmembrane segments. Using one such amplified fragment as a probe, we cloned a novel member of the G protein-coupled receptor superfamily in Xenopus. Alignment of the deduced protein sequence with that of other receptors discloses some homology with angiotensin receptors. A single transcript of 2.5 kb is detected at the late blastula stage and its expression increases during gastrulation. In situ hybridization reveals transcripts initially in the ventrolateral involuting marginal zone and later in the lateral plate mesoderm. At larval stages, the transcript is expressed in procardiac tube and forming blood vessels, where it is localized in the inner endothelial layer. Thus, this gene traces an endothelial lineage and represents a very early and unique marker in Xenopus of the specification of cardiac and vascular endothelia. We propose the name of X-msr for mesenchyme-associated serpentine receptor.