Role for phagocytosis in the prevention of neoplastic transformation in Drosophila
Role for phagocytosis in the prevention of neoplastic transformation in Drosophila
复制标题
吞噬作用在预防果蝇肿瘤转化中的作用
DOI：
10.1111/gtc.12804
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发表时间：
2020
期刊：
影响因子：
2.1
通讯作者：
Nakanishi Yoshinobu
中科院分区：
文献类型：
--
作者：
Zhang Min;Nagaosa Kaz;Nakai Yuji;Yasugi Tetsuo;Kushihiki Masako;Rahmatika Dini;Sato Makoto;Shiratsuchi Akiko;Nakanishi Yoshinobu
免疫力被认为与癌症的预防有关。虽然体液免疫和细胞免疫反应都可能参与其中，但其潜在机制尚未阐明。本研究使用果蝇模型来阐明这一问题，在该模型中，通过同时抑制细胞周期检查点和细胞凋亡来诱导肿瘤转化。我们首先确定了血细胞的位置，果蝇的血细胞发挥免疫细胞的作用，在肿瘤诱导和正常幼虫，但两组之间没有显着差异。当确定幼虫血细胞中的基因表达模式时，免疫相关基因（包括吞噬作用所必需的基因）的表达在瘤形成模型中降低。然后，我们询问吞噬作用在肿瘤预防中的参与，检查吞噬受体而不是细胞凋亡的表达被延迟的动物。我们发现，抑制吞噬受体表达增加了细胞周期检查点缺陷诱导的瘤形成的发生。这表明吞噬作用在果蝇的肿瘤转化预防中的作用。
Immunity is considered to be involved in the prevention of cancer. Although both humoral and cellular immune reactions may participate, underlying mechanisms have yet to be clarified. The present study was conducted to clarify this issue using aDrosophilamodel, in which neoplastic transformation was induced through the simultaneous inhibition of cell‐cycle checkpoints and apoptosis. We first determined the location of hemocytes, blood cells ofDrosophilaplaying a role of immune cells, in neoplasia‐induced and normal larvae, but there was no significant difference between the two groups. When gene expression pattern in larval hemocytes was determined, the expression of immunity‐related genes including those necessary for phagocytosis was reduced in the neoplasia model. We then asked the involvement of phagocytosis in the prevention of neoplasia examining animals where the expression of engulfment receptors instead of apoptosis was retarded. We found that the inhibition of engulfment receptor expression augmented the occurrence of neoplasia induced by a defect in cell‐cycle checkpoints. This suggested a role for phagocytosis in the prevention of neoplastic transformation inDrosophila.