Biochemical Characterization of Collagen in Alveolar Mucosa and Attached Gingiva of Pig
Biochemical Characterization of Collagen in Alveolar Mucosa and Attached Gingiva of Pig
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DOI：
10.1080/03008200601143508
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发表时间：
2007-01
影响因子：
2.9
通讯作者：
Michitsuna Katafuchi;T. Matsuura;P. Atsawasuwan;Hironobu Sato;M. Yamauchi
中科院分区：
文献类型：
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作者：
Michitsuna Katafuchi;T. Matsuura;P. Atsawasuwan;Hironobu Sato;M. Yamauchi
牙槽粘膜和附着的牙龈是覆盖颌骨牙槽骨的两个连续但功能不同的结缔组织。在这项研究中，这些组织的主要基质成分，胶原蛋白，进行了生化表征和比较。从成熟猪获得组织，并分析胶原含量、氨基酸组成、胶原类型、胶原交联和基因表达。我们发现，牙槽粘膜主要由纤维状胶原组成，胶原含量高于附着牙龈。III型胶原相对于I型胶原的含量在牙槽粘膜中高于附着牙龈。两种组织之间的胶原交联模式也是不同的，这表明与附着的牙龈相比，牙槽粘膜含有较少的可还原交联，但含有更多的不可还原交联。Ⅰ型胶原mRNA在牙槽黏膜中的表达水平明显低于附着龈。这些结果表明，肺泡粘膜是一种纤维状的胶原丰富的组织，与牙龈组织相比，重建缓慢。
Alveolar mucosa and attached gingiva are two continuous but functionally distinct connective tissues covering alveolar bone of the jaw. In this study, the major matrix component of these tissues, collagen, was biochemically characterized and compared. The tissues were obtained from mature pigs and analyzed for collagen content, amino acid composition, collagen types, collagen cross-linking, and gene expression. We found that alveolar mucosa is primarily composed of fibrillar collagens and the collagen content is higher than attached gingiva. The content of type III relative to type I collagen was higher in alveolar mucosa when compared with attached gingiva. The collagen cross-linking pattern also was distinct between the two tissues demonstrating that alveolar mucosa contained fewer reducible cross-links but more nonreducible cross-links in comparison to attached gingiva. The mRNA expression level of type I collagen in alveolar mucosa was significantly lower than that of attached gingiva. These results indicate that alveolar mucosa is a fibrillar collagen-rich tissue and, in comparison to gingival tissue, remodels slowly.