Novel lymphography using indocyanine green dye for near-infrared fluorescence labeling
Novel lymphography using indocyanine green dye for near-infrared fluorescence labeling
复制标题
DOI：
10.1097/01.sap.0000250896.42800.a2
复制
发表时间：
2007-06-01
影响因子：
1.5
通讯作者：
Morimoto, Yuji
中科院分区：
文献类型：
--
作者：
Ogata, Fusa;Azuma, Ryuichi;Morimoto, Yuji
已知淋巴水肿是由许多病理条件引起的;然而，其诊断和治疗策略仍未建立。在这项研究中，我们研究了一种新的淋巴造影方法的实用性的基础上荧光传感使用吲哚菁绿色（ICG）染料成像淋巴管大鼠模型。实时成像系统能够以33帧/秒的速度可视化直径为0.1 mm的浅表淋巴管。此外，即使在潜伏期也检测到辐射诱导的水肿模型中淋巴管的形态学变化。这些结果表明，这种成像技术作为淋巴系统的评价方法是可以接受的。
Lymphedema is known to be caused by many pathologic conditions; however, its diagnostic and therapeutic strategies remain to be unestablished. In this study, we investigated the usefulness of a novel lymphographic method based on fluorometric sensing using indocyanine green (ICG) dye for imaging lymphatic vessels using rat models. The real-time imaging system enabled visualization of superficial lymphatic vessels with a diameter of 0.1 mm in 33 frames/second. In addition, morphologic changes in lymphatic vessels in a radiation-induced lymphedema model were detected even at the latent stage. These results suggest that this imaging technique is acceptable as an evaluation method for the lymphatic system.