Movement of retinal along the visual transduction path
Movement of retinal along the visual transduction path
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DOI：
10.1126/science.288.5474.2209
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发表时间：
2000-06-23
期刊：
影响因子：
56.9
通讯作者：
Nakanishi, K
中科院分区：
文献类型：
--
作者：
Borhan, B;Souto, ML;Nakanishi, K
视紫红质 (Rh) 中配体/受体复合物的运动已被追踪。含有 11-cis-3-diazo-4-oxo-retinal 的重氮酮视紫红质 (DK-Rh) 的漂白可产生与天然 kh 相对应的 Batho-、lumi-、meta-I- 和 meta-II-Rh 中间体，但温度较低。 DK-Rh 和这些漂白中间体的光亲和标记表明，DK-Rh 和 Batho-Rh 中紫罗兰酮环与螺旋 F 上的色氨酸-265 交联，以及 lumi-、meta-I- 和 meta-II-Rh 中间体中螺旋 D 上的丙氨酸-169 交联。很可能这些涉及发色环翻转的运动引发了 细胞质膜环导致异三聚鸟嘌呤核苷酸结合蛋白（G 蛋白）激活。
Movement of the ligand/receptor complex in rhodopsin (Rh) has been traced. Bleaching of diazoketo rhodopsin (DK-Rh) containing 11-cis-3-diazo-4-oxo-retinal yields batho-, lumi-, meta-I-, and meta-II-Rh intermediates corresponding to those of native kh but at lower temperatures. Photoaffinity labeling of DK-Rh and these bleaching intermediates shows that the ionone ring cross-links to tryptophan-265 on helix F in DK-Rh and batho-Rh, and to alanine-169 on helix D in lumi-, meta-I-, and meta-II-Rh intermediates, It is likely that these movements involving a flip-over of the chromophoric ring trigger changes in cytoplasmic membrane loops resulting in heterotrimeric guanine nucleotide-binding protein (G protein) activation.