Effect of thermal manipulation of broilers embryos on the response to heat-induced oxidative stress
Effect of thermal manipulation of broilers embryos on the response to heat-induced oxidative stress
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DOI：
10.3382/ps/pey379
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发表时间：
2019-02-01
期刊：
影响因子：
4.4
通讯作者：
Ababneh, M. M.
中科院分区：
文献类型：
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作者：
Al-Zghoul, M. B.;Sukker, H.;Ababneh, M. M.
研究了胚胎热操作 (TM) 对 2 个肉鸡品种中热诱导氧化应激相关基因（NOX4、GpX2、SOD2、过氧化氢酶和 AvUCP）的 mRNA 表达水平和总抗氧化能力的影响。受精的 Cobb 和 Hubbard 蛋 (n = 1,200) 被分为 4 个处理组：Cobb 对照、Cobb TM、Hubbard 对照和 Hubbard TM。对照组维持在标准条件（37.8摄氏度；56%相对湿度）下，而TM组从胚胎第10天（ED）到第18天，每天在39摄氏度和65%相对湿度下孵化18小时。在孵化后第28天，肉鸡在40摄氏度下承受急性热应激（AHS）7小时。每隔一定时间（0、1、3、5和7小时），对4组各12只鸡进行人道安乐死，并立即分离肝脏样本。 AHS期间，两个品种TM组鸡的NOX4、GPx2、SOD2和过氧化氢酶的mRNA表达水平均显着低于对照组，但TM组的AvUCP mRNA表达量较高。两个品种的TM组总抗氧化能力以及超氧化物酶歧化酶和过氧化氢酶活性均显着低于对照组。这项研究的结果表明，TM 对 2 个肉鸡品种的耐热性获得具有长期影响，与热诱导氧化应激相关的系统基因的减少表明了这一点。
Effects of embryonic thermal manipulation (TM) on mRNA expressional levels and total antioxidant capacity of genes associated with heat-induced oxidative stress (NOX4, GpX2, SOD2, catalase, and AvUCP) in 2 breeds of broiler chicken were investigated. Fertile Cobb and Hubbard eggs (n = 1,200) were divided into 4 treatment groups: Cobb control, Cobb TM, Hubbard control, and Hubbard TM. Control groups were maintained under standard conditions (37.8 degrees C; 56% relative humidity), whereas TM groups were incubated at 39 degrees C and 65% relative humidity for 18 h a day from embryonic days (ED) 10 to 18. On post-hatch day 28, the broilers were subject to acute heat stress (AHS) at 40 degrees C for 7 h. At certain intervals (0, 1, 3, 5, and 7 h), 12 chickens from each of the 4 groups were humanely euthanized, and liver samples were immediately isolated. During AHS, in both breeds, the mRNA expression levels of NOX4, GPx2, SOD2, and catalase in TM chickens were significantly lower than in controls, but AvUCP mRNA expression in the TM group was higher. The total antioxidant capacity and activity of superoxidase dismutase and catalase were significantly lower in the TM than in the control group in both breeds. The results of this study suggest that TM has a long-lasting effect on the acquisition of thermotolerance in 2 broiler chicken breeds as indicated by the reduction of system genes associated with heat induced oxidative stress.