An immunocytochemical analysis of the expression of thyroid hormone receptor α and β proteins during natural and thyroid hormone‐induced metamorphosis in Xenopus
An immunocytochemical analysis of the expression of thyroid hormone receptor α and β proteins during natural and thyroid hormone‐induced metamorphosis in Xenopus
复制标题
非洲爪蟾自然变态和甲状腺激素诱导变态过程中甲状腺激素受体 α 和 β 蛋白表达的免疫细胞化学分析
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
J. Tata
中科院分区：
文献类型：
--
作者：
L. Fairclough;J. Tata
两栖动物变态的特征是在自然发育和甲状腺激素（TH）诱导的发育过程中，蝌蚪所有组织中甲状腺激素受体（TR）mRNA的上调。两个TR基因，称为α和β，是与癌基因c-erbA的细胞同源物相关的核受体的大的多基因家族的成员。β亚型的上调现象比α亚型更显著。为了确定转录本的自诱导是否被TR蛋白的自诱导所取代，制备了针对非洲爪蟾TRα和β（xTRα，β）的非交叉反应单克隆抗体，以免疫细胞化学分析其表达和定位。研究了三种蝌蚪组织，它们涵盖了自然和TH诱导变态期间基因重编程的三个主要后果：（i）经历广泛功能转换的肝脏;（ii）在主要结构和生化修饰之前表现出大量细胞死亡的小肠上皮;和（iii）作为从头形态发生示例的后肢芽。结果表明，xTRα蛋白在这些组织中通常更丰富，并且其表达在发育和生殖方面比xTRβ受调控更少。在对变态前（第52阶段）蝌蚪和自然变态开始（第55阶段）给予三碘甲腺原氨酸（T3）后5天，xTRβ的自动诱导特别强烈。在两个阶段的发育中后肢芽中，TRβ的上调受到拓扑学限制，在密集的细胞袋中特别强烈，可能富含软骨细胞。得出的结论是，xTRα和β蛋白的分布和表达部分匹配，但不完全匹配，在自然和激素诱导的变态过程中的转录本。
Amphibian metamorphosis is characterized by the upregulation of thyroid hormone receptor (TR) mRNA in all tissues of tadpole during both the natural and thyroid hormone (TH)‐induced development. The two TR genes, termed α and β, are members of a large multigene family of nuclear receptors related to the cellular homolog of the oncogene c‐erbA. The phenomenon of upregulation is more marked for the β than the α isoform. To determine whether or not the auto‐induction of the transcripts is paralleled by that of TR proteins, non‐cross‐reacting monoclonal antibodies were prepared against Xenopus laevis TRα and β (xTRα, β) in order to analyze immunocytochemically their expression and localization. Three tadpole tissues that exemplify three major consequences of gene re‐programing during natural and TH‐induced metamorphosis were studied: (i) Liver that undergoes extensive functional switching; (ii) small intestinal epithelium that exhibits substantial cell death prior to major structural and biochemical modifications; and (iii) hind limb‐bud as an example of de novo morphogenesis. It was shown that xTRα protein is generally more abundant in these tissues, and its expression is developmentally and hormonally less regulated, than is xTRβ. The auto‐induction of xTRβ was particularly intense at 5 days after administration of triiodo‐thyronine (T3) to both pre‐metamorphic (stage 52) tadpoles and at the onset of natural metamorphosis (stage 55). In the developing hind limb‐bud at both stages the upregulation of TRβ is topologically restricted, being particularly intense in dense pockets of cells, presumably rich in chondrocytes. It was concluded that the distribution and expression of xTRα and β proteins match partially, but not fully, those of their transcripts during natural and hormone‐induced metamorphosis.
影响因子：
11.4
作者：
KARIM, FD;THUMMEL, CS
通讯作者：
THUMMEL, CS
分离编码具有生物活性的甲状腺激素受体的 cDNA 克隆。
DOI：
10.1073/pnas.85.14.5031
发表时间：
1988
影响因子：
11.1
作者：
Koenig,RJ;Warne,RL;Brent,GA;Harney,JW;Larsen,PR;Moore,DD
通讯作者：
Moore,DD
大鼠中枢神经系统中甲状腺激素受体β2样免疫反应性的免疫细胞化学描绘。
影响因子：
4.8
作者：
Lechan,RM;Qi,Y;Berrodin,TJ;Davis,KD;Schwartz,HL;Strait,KA;Oppenheimer,JH;Lazar,MA
通讯作者：
Lazar,MA