Effect of fluoridation of hydroxyapatite in hydroxyapatite-polycaprolactone composites on osteoblast activity
Effect of fluoridation of hydroxyapatite in hydroxyapatite-polycaprolactone composites on osteoblast activity
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DOI：
10.1016/j.biomaterials.2004.11.008
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发表时间：
2005-07-01
期刊：
影响因子：
14
通讯作者：
Knowles, JC
中科院分区：
文献类型：
--
作者：
Kim, HW;Lee, EJ;Knowles, JC
将氟施加到羟基磷灰石（HA）/聚己内酯（PCL）陶瓷-聚合物生物活性复合材料系统中，用于硬组织再生。对羟基磷灰石（HA）进行不同程度的氟化（5%、25%、50%和75%），制备了氟羟基磷灰石（FHA）/PCL复合材料。从细胞贴壁、增殖和分化以及骨相关基因表达等方面观察成骨细胞对复合材料的反应。从复合材料中释放的氟的量通过改变氟化程度来控制，并且细胞反应强烈地受氟化水平的影响。MG 63细胞在FHA-PCL上的附着和增殖水平与HAPCL上的相似。然而，氟化的HA显着增加碱性磷酸酶（ALP）的活性和骨钙素（OC）的生产由复合材料上的细胞，这是通过酶法测定。逆转录-聚合酶链反应（RT-PCR）分析证实，FHA-PCL可上调细胞内ALP和OC的基因表达水平。这些研究结果表明，氟在刺激骨源性细胞活性方面起着重要作用，并且FHA-PCL复合材料具有用作硬组织再生剂的高潜力。(c)2004 Elsevier Ltd.保留所有权利。
Fluorine was administered to a system of hydroxyapatite (HA)/polycaprolactone (PCL) ceramic-polymer bioactive composites for applications as hard tissue regeneratives. The HA was fluoridated at different levels (5%, 25%, 50% and 75%) in order to produce the fluor-hydroxyapatite (FHA)/PCL composites. The osteoblastic cellular responses to the composites were examined in terms of the cell attachment, proliferation and differentiation as well as the expression of bone-associated genes. The amount of fluorine released from the composites was controlled by changing the degree of fluoridation, and the cellular responses were strongly influenced by the level of fluoridation. The MG63 cells on the FHA-PCL attached and proliferated at a similar level to those on HAPCL. However, the fluoridation of HA increased significantly the alkaline phosphatase (ALP) activity and osteocalcin (OC) production by the cells on the composites, which was measured by an enzymatic assay. Moreover, the gene expression level of ALP and OC in the cells was up regulated on the FHA-PCL, which was confirmed semi-quantitatively by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. These findings on the fluorine-administered biological composites (FHA-PCL) suggested that fluorine plays a significant role in stimulating the bone derived cellular activity, and the FHA-PCL composites have high potential for use as hard tissue regeneratives. (c) 2004 Elsevier Ltd. All rights reserved.