Emergence of a multidrug-resistant clone (ST320) among invasive serotype 19A pneumococci in Spain
Emergence of a multidrug-resistant clone (ST320) among invasive serotype 19A pneumococci in Spain
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DOI：
10.1093/jac/dkp210
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发表时间：
2009-09-01
影响因子：
5.2
通讯作者：
Linares, Josefina
中科院分区：
文献类型：
--
作者：
Ardanuy, Carmen;Rolo, Dora;Linares, Josefina
目的：19A血清型肺炎链球菌的多重耐药株近年来在世界各地出现。本研究的目的是分析1997-2007年间在西班牙采集的侵袭性疾病患者的19A血清型肺炎链球菌对青霉素高度耐药的特性。对所有耐青霉素肺炎球菌进行PFGE分型，并对筛选出的菌株进行多位点序列分型(MLST)。结果：西班牙参考实验室1997年至2007年共分离出1 197株19A血清型侵袭性肺炎球菌，其中51株(4.3%)对青霉素高度耐药(MIC2~4 mg/L)。这51株菌分属3个多抗克隆，分别与序列类型(ST)81(n=21)、ST320(n=19)和ST276(n=11)相关。51株19A型肺炎球菌对四环素耐药，携带tet(M)基因；41株对大环内酯类耐药，携带erm(B)基因。与Tn916家族其他基因相关的所有菌株均存在int和xis基因。结论：西班牙对青霉素耐药血清19A侵袭性肺炎球菌的增加与两个全球播散性多重耐药克隆(ST276和ST320)的出现和克隆性传播有关。西班牙(23F)-1-19A(ST81)克隆在整个研究期间保持稳定。多药耐药与Tn916家族转座子相关。
Objectives: Multidrug-resistant Streptococcus pneumoniae isolates of serotype 19A have emerged all over the world in recent years. The aim of this study was to characterize highly penicillin-resistant pneumococcal strains of the 19A serotype, collected in Spain from 1997 to 2007 from patients with invasive disease.Methods: Antibiotic susceptibility was studied by microdilution. All penicillin-resistant pneumococci were typed by PFGE and selected strains were studied by multilocus sequence typing (MLST). The presence of genes related to the Tn916 family of transposons was investigated by PCR.Results: From a total of 1197 invasive pneumococcal isolates of serotype 19A received at the Spanish Reference Laboratory between 1997 and 2007, 51 (4.3%) strains showed high-level resistance to penicillin (MICs of 2-4 mg/L). These 51 isolates belonged to three multiresistant clones related to sequence type (ST) 81 (n=21), ST320 (n=19) and ST276 (n=11). All 51 serotype 19A pneumococci were tetracycline-resistant and had the tet(M) gene, and 41 strains were macrolide-resistant, harbouring the erm(B) gene. The presence of int and xis genes was detected in all strains associated with other genes of the Tn916 family.Conclusions: The rise in penicillin-resistant serotype 19A invasive pneumococci in Spain was associated with the emergence and clonal spread of two worldwide-disseminated multiresistant clones (ST276 and ST320). The Spain(23F)-1-19A (ST81) clone remained stable throughout the study period. Multidrug resistance was associated with transposons of the Tn916 family.