Knockout of Selenoprotein V Affects Regulation of Selenoprotein Expression by Dietary Selenium and Fat Intakes in Mice
Knockout of Selenoprotein V Affects Regulation of Selenoprotein Expression by Dietary Selenium and Fat Intakes in Mice
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DOI：
10.1093/jn/nxz287
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发表时间：
2020-03-01
影响因子：
4.2
通讯作者：
Lei, Xin Gen
中科院分区：
文献类型：
--
作者：
Chen, Ling-Li;Huang, Jia-Qiang;Lei, Xin Gen
背景：硒蛋白V （SELENOV）的代谢功能尚不清楚。目的：通过两项实验，研究硒基因敲除（Selenov基因敲除）对饲喂不同硒和脂肪浓度小鼠血清、肝脏、睾丸和/或白色脂肪组织（VAT）中硒浓度及4种主要硒蛋白Iglutathione过氧化物酶（GPX） 1、GPX4、硫氧还蛋白还原酶-1 （TXNRD1）和硒蛋白P (SELENOP)] mRNA、蛋白和/或活性的影响。方法：在实验（Expt） 1中，40只KO和40只野生型（WT）小鼠（雄性，8周龄）（n = 10/基因型）分别饲喂酪蛋白-蔗糖基础饲粮，外加0、0.3、1或3 mg硒/kg（亚硒酸钠），持续32周。在实验2中，20只KO和20只WT小鼠（雄性，8周龄）被喂食（n = 10/基因型）正常脂肪饮食（NF； 10%的热量来自脂肪）或高脂肪饮食（HF； 60%的热量来自脂肪）19周。结果：在实验1中，KO引起睾丸Gpxl、Txnrdl、Selenop mRNA量一致或显著降低（P < 0.05） (
Background: The metabolic function of selenoprotein V (SELENOV) remains unknown.Objectives: Two experiments were conducted to determine effects of the Selenov knockout (KO) on selenium concentration and mRNA, protein, and/or activity of 4 major selenoproteins Iglutathione peroxidase (GPX) 1, GPX4, thioredoxin reductase-1 (TXNRD1), and selenoprotein P (SELENOP)] in the serum, liver, testis, and/or white adipose tissue (VAT) of mice fed different dietary selenium and fat concentrations.Methods: In Experiment (Expt) 1, 40 KO and 40 wild-type (WT) mice (males, 8 wk old) were fed (n = 10/genotype) a casein-sucrose basal diet plus 0, 0.3, 1, or 3 mg Se/kg (as sodium selenite) for 32 wk . In Expt 2, 20 KO and 20 WT mice (males, 8 wk old) were fed (n = 10/genotype) a normal-fat diet (NF; 10% calories from fat) or a high-fat diet (HF; 60% calories from fat) for 19 wk.Results: In Expt 1, the KO caused consistent or substantial decreases (P < 0.05) of mRNA amounts of Gpxl, Txnrdl, and Selenop in the testis (